In this study, simple, new and functional silky nanostructures of Co₃O₄ are prepared by hydrothermal method. These nanostructures are successfully used for the enzyme free sensing of uric acid in 0.1 M phosphate buffer solution of pH 7.3. Physical characterization experiments were carried out to explore the morphology, composition and crystalline phase of the newly prepared Co₃O₄ nanostructures. Scanning electron microscopy (SEM) shows a silk like morphology and energy dispersive spectroscopy (EDS) revealed the presence of Co and O as the main elements. Powder X-ray diffraction (XRD) demonstrates a cubic crystallography with well resolved diffraction patterns. The electrochemical activity of these silky Co₃O₄ nanostructures was evaluated by cyclic voltammetry (CV) in a 0.1 M phosphate buffer solution at pH 7.3. The high purity and unique morphology of Co₃O₄ shows a highly sensitive and selective response towards the non-enzymatic sensing of uric acid. This uric acid sensor exhibits a linear range of 0.5 mM to 3.5 mM uric acid and a 0.1 mM limit of detection. The anti-interference capability of this uric acid sensor was monitored in the presence of common interfering species. Furthermore, electrochemical impedance spectroscopy confirms a low charge transfer resistance value of 5.11 K Ω cm² for silky Co₃O₄ nanostructures which significantly supported the CV results. The proposed modified electrode is stable, selective and reproducible which confirms its possible practical use. Silky Co₃O₄ nanostructures can be of great importance for diverse electrochemical applications due to their excellent electrochemical activity and large surface area.