Issue 13, 2021, Issue in Progress
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RSC Advances

A straightforward and sensitive “ON–OFF” fluorescence immunoassay based on silicon-assisted surface enhanced fluorescence†

Ruohu Zhang,a   Zhanrui Jin,a   Zhengqiu Tian,a   Yingzhou Liu,a   Zhengqi Lua  and  Yiping Cui ORCID logo *a  

* Corresponding authors

a Advanced Photonics Center, Southeast University, Nanjing 210096, Jiangsu, China
E-mail: cyp@seu.edu.cn

Abstract

A straightforward immunoassay based on silicon-assisted surface enhanced fluorescence (SEF) has been demonstrated using a silicon-based fluorescent immune substrate and silver-antibody nanoconjugate (SANC). The P-doped, (100) oriented silicon wafers are used for both fluorophore attachment and antigen immobilization. The silicon substrate offers a very low blank signal in the “OFF” state, due to its fluorescence quenching effect. In the detection process, the capture of the SANCs by the surface-immobilized antigen leads to an effectively enhanced fluorescence to produce an “ON” state. The analytical performance of the presented scheme has been investigated and a limit of detection of 31.4 pg mL−1 has been obtained. Besides the broadened application range compared with the conventional immunoassays, the presented scheme is straightforward, cost effective and sensitive, and is hence expected to find widespread applications in immunoassays as well as other fluorescence-based assays.

Graphical abstract: A straightforward and sensitive “ON–OFF” fluorescence immunoassay based on silicon-assisted surface enhanced fluorescence

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Article information

DOI
https://doi.org/10.1039/D0RA08759A
Article type
Paper
Submitted
14 Oct 2020
Accepted
08 Feb 2021
First published
17 Feb 2021
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2021,11, 7723-7731

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A straightforward and sensitive “ON–OFF” fluorescence immunoassay based on silicon-assisted surface enhanced fluorescence

R. Zhang, Z. Jin, Z. Tian, Y. Liu, Z. Lu and Y. Cui, RSC Adv., 2021, 11, 7723 DOI: 10.1039/D0RA08759A

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