Issue 44, 2020, Issue in Progress

A mitochondrion-targeted dual-site fluorescent probe for the discriminative detection of SO32− and HSO3 in living HepG-2 cells

Abstract

Sulfur dioxide, known as an environmental pollutant, produced during industrial productions is also a common food additive that is permitted worldwide. In living organisms, sulfur dioxide forms hydrates of sulfite (SO2·H2O), bisulfite (HSO3) and sulfite (SO32−) under physiological pH conditions; these three exist in a dynamic balance and play a role in maintaining redox balance, further participating in a wide range of physiological and pathological processes. On the basis of the differences in nucleophilicity between SO32− and HSO3, for the first time, we built a mitochondrion-targeted dual-site fluorescent probe (Mito-CDTH-CHO) based on benzopyran for the highly specific detection of SO32− and HSO3 with two diverse emission channels. Mito-CDTH-CHO can discriminatively respond to the levels of HSO3 and SO32−. Besides, its advantages of low cytotoxicity, superior biocompatibility and excellent mitochondrial enrichment ability contribute to the detection and observation of the distribution of sulfur dioxide derivatives in living organisms as well as allowing further studies on the physiological functions of sulfur dioxide.

Graphical abstract: A mitochondrion-targeted dual-site fluorescent probe for the discriminative detection of SO32− and HSO3− in living HepG-2 cells

Supplementary files

Article information

Article type
Paper
Submitted
10 Feb 2020
Accepted
28 Jun 2020
First published
14 Jul 2020
This article is Open Access
Creative Commons BY license

RSC Adv., 2020,10, 26349-26357

A mitochondrion-targeted dual-site fluorescent probe for the discriminative detection of SO32− and HSO3 in living HepG-2 cells

Z. Deng, F. Li, G. Zhao, W. Yang and Y. Hu, RSC Adv., 2020, 10, 26349 DOI: 10.1039/D0RA01233E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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