Issue 15, 2021

Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe

Abstract

Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in live Escherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.

Graphical abstract: Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe

Supplementary files

Article information

Article type
Communication
Submitted
26 Oct 2020
Accepted
23 Dec 2020
First published
23 Dec 2020

Chem. Commun., 2021,57, 1875-1878

Assessing changes in the expression levels of cell surface proteins with a turn-on fluorescent molecular probe

J. Hatai, P. K. Prasad, N. Lahav-Mankovski, N. Oppenheimer-Low, T. Unger, Y. F. Sirkis, T. Dadosh, L. Motiei and D. Margulies, Chem. Commun., 2021, 57, 1875 DOI: 10.1039/D0CC07095E

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