Issue 58, 2020
From the journal:

Chemical Communications

An NMR based phosphodiesterase assay†

Madoka Akimoto, ORCID logo a   Tianning Yu,b   Kody Moleschi,a   Katherine Van,b   Ganesh S. Anandc  and  Giuseppe Melacini ORCID logo *ab  

* Corresponding authors

a Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Canada
E-mail: melacin@mcmaster.ca

b Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Canada

c Department of Biological Sciences, National University of Singapore, Singapore

Abstract

We propose a phosphodiesterase assay based on 1D 1H NMR to monitor the hydrolysis of cyclic nucleotides directly, without requiring tags or the addition of exogenous reagents. The method is suitable to measure phosphodiesterase KM and kcat parameters and to identify phosphodiesterase inhibitors.

Graphical abstract: An NMR based phosphodiesterase assay

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Article information

DOI
https://doi.org/10.1039/D0CC01673J
Article type
Communication
Submitted
03 Mar 2020
Accepted
01 Jun 2020
First published
17 Jun 2020

Chem. Commun., 2020,56, 8091-8094

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An NMR based phosphodiesterase assay

M. Akimoto, T. Yu, K. Moleschi, K. Van, G. S. Anand and G. Melacini, Chem. Commun., 2020, 56, 8091 DOI: 10.1039/D0CC01673J

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