Issue 11, 2019
From the journal:

Food & Function

GABA attenuates ETEC-induced intestinal epithelial cell apoptosis involving GABAAR signaling and the AMPK-autophagy pathway†

Yaoyao Xia,a   Siyuan Chen,a   Yuanyuan Zhao,a   Shuai Chen, ORCID logo bc   Ruilin Huang,b   Guoqiang Zhu,d   Yulong Yin,ab   Wenkai Ren ORCID logo *ad  and  Jinping Deng*a  

* Corresponding authors

a Guangdong Provincial Key Laboratory of Animal Nutrition Control, Institute of Subtropical Animal Nutrition and Feed, College of Animal Science, South China Agricultural University, Guangzhou, China
E-mail: renwenkai19@126.com, dengjinping@scau.edu.cn

b Laboratory of Animal Nutrition and Health and Key Laboratory of Agro-Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China

c University of Chinese Academy of Sciences, Beijing, China

d Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, College of Veterinary Medicine, Yangzhou University, Yangzhou, China

Abstract

Enterotoxigenic Escherichia coli (ETEC) triggers diarrhea in humans and livestock. We have previously showed that ETEC promotes intestinal epithelial cell apoptosis and increases gamma-aminobutyric acid (GABA) concentration in the jejunum, suggesting that GABA might mediate ETEC-induced apoptosis. Here, we found that GABA alleviates ETEC-induced intestinal barrier dysfunctions, including ETEC-induced apoptosis both in vivo and in vitro. Interestingly, the alleviation of GABA on ETEC-induced apoptosis largely depends on autophagy. Mechanistically, GABA attenuates ETEC-induced apoptosis via activating GABAAR signaling and the AMPK-autophagy pathway. These findings highlight that maintaining intestinal GABA concentration could alleviate intestinal ETEC infection.

Graphical abstract: GABA attenuates ETEC-induced intestinal epithelial cell apoptosis involving GABAAR signaling and the AMPK-autophagy pathway

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Article information

DOI
https://doi.org/10.1039/C9FO01863H
Article type
Paper
Submitted
16 Aug 2019
Accepted
05 Oct 2019
First published
09 Oct 2019

Food Funct., 2019,10, 7509-7522

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GABA attenuates ETEC-induced intestinal epithelial cell apoptosis involving GABAAR signaling and the AMPK-autophagy pathway

Y. Xia, S. Chen, Y. Zhao, S. Chen, R. Huang, G. Zhu, Y. Yin, W. Ren and J. Deng, Food Funct., 2019, 10, 7509 DOI: 10.1039/C9FO01863H

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