Issue 14, 2019
From the journal:

Analyst

Fluorescence polarization-based detection of cancer-related mutations using target-initiated rolling circle amplification†

Woo Young Kwon, ORCID logo a   Byung Seok Cha,a   Seokjoon Kim,a   Sung Hyun Hwang,a   Ji Min Kim,a   Kalishwaralal Kalimuthu,a   Hyun Gyu Park ORCID logo b  and  Ki Soo Park ORCID logo *a  

* Corresponding authors

a Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea
E-mail: kskonkuk@gmail.com, akdong486@konkuk.ac.kr
Fax: +82-2-450-3742
Tel: +82-2-450-3742

b Department of Chemical and Biomolecular Engineering (BK 21+ program), KAIST, Daehak-ro 291, Yuseong-gu, Daejeon 34141, Republic of Korea

Abstract

We devised a new method to detect cancer-related mutations based on target-initiated rolling circle amplification in combination with fluorescence polarization. We then applied this method to identify the presence of KRAS G13D and G12D, two of the most frequent mutations found in colorectal cancer patients, demonstrating high sensitivity and specificity.

Graphical abstract: Fluorescence polarization-based detection of cancer-related mutations using target-initiated rolling circle amplification

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Article information

DOI
https://doi.org/10.1039/C9AN00429G
Article type
Communication
Submitted
06 Mar 2019
Accepted
11 Jun 2019
First published
18 Jun 2019

Analyst, 2019,144, 4149-4152

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Fluorescence polarization-based detection of cancer-related mutations using target-initiated rolling circle amplification

W. Y. Kwon, B. S. Cha, S. Kim, S. H. Hwang, J. M. Kim, K. Kalimuthu, H. G. Park and K. S. Park, Analyst, 2019, 144, 4149 DOI: 10.1039/C9AN00429G

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