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Ultraviolet A-induced hematotoxic and genotoxic potential in Nile tilapia Oreochromis niloticus

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Abstract

Fish as an aquatic organism could be harmed by various levels of solar ultraviolet radiation (UVA). The present study aimed to characterize UVA (20, 60 and 180 min for 3 days) irradiation-induced hematological and biochemical changes in Oreochromis niloticus. Hematological parameters such as the red blood cell (RBC) count, hemoglobin (Hb) level and hematocrit (Hct) value were significantly (p < 0.05) reduced in fish exposed to different doses of UVA. Also, the leukocyte (WBC) count was significantly reduced (p < 0.05). However, the differential counts of WBCs - lymphocytes, eosinophils and monocytes - increased significantly in the exposed fish compared to the control fish. Many morphological and genotoxic alterations in erythrocytes were observed in the present work. The glucose level showed a significant decrease, but cholesterol and triglycerides showed a significant increase after exposure to UVA. Total protein levels of the fish showed a significant increase (p < 0.05) in the exposed groups. Also, concentrations of urea and creatinine increased significantly as fish were exposed to increasing UVA radiation, compared to the control fish. Finally, the activities of alanine aminotransferase (ALT, U l−1) and aspartate aminotransferase (AST, U l−1) exhibited a significant increase (p < 0.05) with increasing UVA doses.

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References

  1. M. P. Chipperfield, S. Bekki, S. Dhomse, N. R. P. Harris, B. Hassler, R. Hossaini, W. Steinbrecht, R. Thiéblemont and M. Weber, Nature, 2017, 549, 211–218.

    CAS  PubMed  Google Scholar 

  2. M. Blumthaler and A. R. Webb, UVR climatology, in Comprehensive Series in Photochemistry and Photobiology, ed. E.W. Helbling and H.E. Zagarese, Royal Society of Chemistry, 2003, pp. 23–58.

    Google Scholar 

  3. B. R. Hargreaves, Water column optics and penetration of UVR, in Comprehensive Series in Photochemistry and Photobiology, ed. E.W. Helbling and H.E. Zagarese, Royal Society of Chemistry, 2003, pp. 59–105.

    Google Scholar 

  4. M. Tedetti and R. Sempéré, Penetration of Ultraviolet Radiation in the Marine Environment. A Review, Photochem. Photobiol., 2006, 82, 389–397.

    Article  CAS  PubMed  Google Scholar 

  5. M. Vázquez and A. Hanslmeier, Ultraviolet Radiation in the Solar System, Springer, 2006.

    Book  Google Scholar 

  6. A. T. Ibrahim, Negative impacts of ultraviolet-A radiation on antioxidant and oxidative stress biomarkers of African catfish Clarias gariepinus, Photochem. Photobiol. Sciences, 2015, 14, 1337–1345.

    Article  CAS  Google Scholar 

  7. B. Obermueller, S. Puntarulo and D. Abele, UV-tolerance and instantaneous physiological stress responses of two Antarctic amphipod species Gondogeneia antarctica and Djerboa furcipes during exposure to UV radiation, Mar. Environ. Res., 2007, 64, 267–285.

    Article  CAS  Google Scholar 

  8. S. J. V. Dacie and S. M. Lewis, Practical haematology, Churchill Livingstone, UK, 11th edn, 2003.

    Google Scholar 

  9. M. Tavares-Dias and F. R. Moraes, Haematological evaluation of Tilapia rendalli Boulenger, 1896 (Ostrichthyes: Cichlidae) captured in a fee fishing farm in Franca Säo Paulo State, Brazil, Biosci. J., 2003, 19, 103–110.

    Google Scholar 

  10. S. Pascoe and D. Gatehouse, The use of a simple haematoxylin and eosin staining procedure to demonstrate micronuclei within rodent bone marrow, Mutat. Res., Environ. Mutagen. Relat. Subj., 1986, 164, 237–243.

    CAS  Google Scholar 

  11. A. Osman, E. Ali, M. Hashem, M. Mostafa and I. A. A. Mekkawy, Genotoxicity of two pathogenic strains of zoosporic fungi (achlyaklebsianaand aphanomyceslaevis) on erythrocytes of nile tilapia oreochromis niloticus, Ecotoxicol. Environ. Saf., 2010, 73, 24–31.

    Article  CAS  PubMed  Google Scholar 

  12. H. S. Hamed and A. G. M. Osman, Modulatory effect of lycopene against carbofuran toxicity in African catfish, Clarias gariepinus, Fish Physiol. Biochem., 2017, 43, 1721–1731.

    Article  CAS  PubMed  Google Scholar 

  13. A. S. A. Harabawy and A. T. A. Ibrahim, Sublethal toxicity of carbofuran pesticide on the African catfish Clarias gariepi- nus (Burchell, 1822): Hematological, biochemical and cytogenetic response, Ecotoxicol. Environ. Saf., 2014, 103, 61–67.

    Article  CAS  PubMed  Google Scholar 

  14. A. G. M. Osman and A. S. A. Harabawy, Hematotoxic and genotoxic potential of ultraviolet-A radiation on the African catfish Clarias gariepinus (Burchell, 1822), J. Fish. Int., 2010, 5, 44–53.

    Article  Google Scholar 

  15. J. Cejkova, S. Stipek, J. Crkovska and T. Ardan, Changes of superoxide dismutase, cathalase and glutathione peroxidase in the corneal epithelium after UVB rays. Histochemical and biochemical study, Histol. Histopathol., 2000, 15, 1043–1050.

    CAS  PubMed  Google Scholar 

  16. A. T. A. Ibrahim, Negative impacts of ultraviolet-A radiation on antioxidant and oxidative stress biomarkers of African catfish Clarias gariepinus, Photochem. Photobiol. Sci., 2015, 14, 1337–1345.

    Article  CAS  PubMed  Google Scholar 

  17. T. J. McMillan, E. Leatherman, A. Ridley, J. Shorrocks, S. E. Tobi and J. R. Whiteside, Cellular effects of long wavelength UV light (UVA) in mammalian cells, J. Pharm. Pharmacol., 2008, 60, 969–976.

    Article  CAS  PubMed  Google Scholar 

  18. A. H. Sayed, A. T. Ibrahim, I. A. A. Mekkawy and U. M. Mahmoud, Acute effects of Ultraviolet-A radiation on African Catfish Clarias gariepinus (Burchell, 1822), J. Photochem. Photobiol., B, 2007, 89, 170–174.

    Article  CAS  Google Scholar 

  19. E. Markkula, H. M. Salo, K. Rikalainen and I. E. Jokinen, Long-term UVB irradiation affects the immune functions of carp (Cyprinuscarpio) and rainbow trout (Oncorhynchus mykiss), Photochem. Photobiol., 2009, 85, 347–352.

    Article  CAS  PubMed  Google Scholar 

  20. S. E. Markkula, H. M. Salo, A. K. Rikalainen and E. I. Jokinen, Different sensitivity of carp (Cyprinuscarpio) and rainbow trout (Oncorhynchus mykiss) to the immunomodulatory effects of UVB irradiation, Fish Shellfish Immunol., 2006, 21, 70–79.

    Article  CAS  PubMed  Google Scholar 

  21. O. Kori-Siakpere and E. O. Ubogu, Sublethal haematological effects of zinc on the freshwater fish, Heteroclarias sp. (Osteichthyes: Clariidae), Afr.J. Biotechnol., 2008, 7, 2068–2073.

    Article  CAS  Google Scholar 

  22. I. A. Mekkawy, U. M. Mahmoud and R. H. Mohammed, Protective effects of tomato paste and Vitamin E on Atrazine-induced hematological and biochemical characteristics ofClarias gariepinus (Burchell, 1822), Adv. Res. J. Environ. Sci. Toxicol., 2013, 2, 11–21.

    Google Scholar 

  23. B. Magnadottir, Innate immunity of fish (overview), Fish Shellfish Immunol., 2006, 20, 137–151.

    Article  CAS  Google Scholar 

  24. I. E. Jokinen, H. M. Salo, E. Markkula, K. Rikalainen, M. T. Arts and H. I. Browman, Additive effects of enhanced ambient ultraviolet B radiation and increased temperature on immune function, growth and physiological condition of juvenile ( parr) Atlantic Salmon, Salmo salar, Fish Shellfish Immunol., 2011, 30, 102–108.

    Article  CAS  PubMed  Google Scholar 

  25. C. A. Castro-Pérez, Presented in part at the International Congress on the Biology of Fish, Tropical Hotel Resort Manaus Brazil, 2004.

    Google Scholar 

  26. M. Far, S. H. V. Roodsari, A. Zamini, E. Mirrasooli and R. Kazemi, The effects of diazinon on behavior and some hematological parameters of fry rainbow trout (Oncorhynchus mykiss), World J. Fish Mar. Sci., 2012, 4, 369–375.

    CAS  Google Scholar 

  27. M. M. N. Authman, S. A. Ibrahim, M. A. El-Kasheif and H. S. Gaber, Heavy metals pollution & their effects on gills and liver of the nile catfish clarias gariepinus inhabiting el-rahawy drain, Egypt, Global Vet., 2013, 10, 103–115.

    CAS  Google Scholar 

  28. T. B. Kovyrshina and I. I. Rudneva, Seasonal dynamics of activity of oxidative modification of proteins and oxidationinhibiting enzymes in the blood of go by Neogobius melanostomus inhabiting the black sea and the sea ofazov, J. Ichthyol., 2012, 52, 277–283.

    Article  Google Scholar 

  29. M. Saravanan, K. P. Kumar and M. Ramesh, Haematological and biochemical responses of fresh water teleost fish Cyprinuscarpio (actinopterygii. Cypriniformes) during acute and chronic sublethal exposure to lindane, Pestic. Biochem. Physiol., 2011, 100, 206–211.

    Article  CAS  Google Scholar 

  30. S. I. Tayel, S. A. Ibrahim, M. M. N. Authman and M. A. El-Kashef, Assessment of sabal drainage canal water quality and its effect on blood and spleen histology of Oreochromis niloticus, Afr. J. Biol. Sci., 2007, 3, 97–107.

    Google Scholar 

  31. K. Sharf-Eldeen and N. Abdel-Hamid, Sublethal effects of copper sulphate, malathion and paraquat on protein pattern of Oreochromis niloticus, Egypt. J. Aquat. Biol. Fish., 2002, 6, 167–182.

    Article  Google Scholar 

  32. A. M. Shalaby, A. A. Ramadan, M. A. ElGammal, E. M. ElAganif and M. S. M. Ebrahim, Beneficial using of EDTA to reduce cadmium toxicity and to improve the physiological and biochemical profiles of catfish (Clarias gariepinus), Egypt. J. Aquacult., 2011, 1, 55–69.

    Article  Google Scholar 

  33. H. Turker, Haematological Effects Of Ultraviolet-C Radiation On Swiss Albino Mice, Int. Toxicol. Appl. Pharmacol., 2014, 4, 17–22.

    Google Scholar 

  34. C. Rabasa and S. L. Dickson, Impact of stress on metabolism and energy balance, Curr. Opin. Behav. Sci., 2016, 9, 71–77.

    Article  Google Scholar 

  35. B. Kaur, A. Khera and R. Sandhir, Attenuation of cellular antioxidant defense mechanisms in kidney of rats intoxicated with carbofuran, J. Biochem. Mol. Toxicol., 2012, 26, 393–398.

    Article  CAS  PubMed  Google Scholar 

  36. A. C. Abcar, L. Chan and H. Yeoh, What To Do for the Patient with Minimally Elevated Creatinine Level?, Perm. J., 2004, 8, 51–53.

    PubMed  PubMed Central  Google Scholar 

  37. A. G. M. Osman, M. Koutb and A. E. H. Sayed, Use of hematological parameters to assess the efficiency of quince (cydoniaoblonga miller) leaf extract in alleviation of the effect of ultraviolet-A radiation on African catfish Clarias gariepinus (Burchell, 1822), J. Photochem. Photobiol., B, 2010, 99, 1–8.

    Article  CAS  Google Scholar 

  38. S. Barhoumi, I. Messaoudi, F. Gagné and A. Kerkeni, Spatial and seasonal variability of some biomarkers in salariabasilisca(pisces. Blennidae). Implication for biomonitoring in tunisian coasts, Ecol. Indic., 2012, 14, 222–228.

    Article  CAS  Google Scholar 

  39. S. A. Ibrahim and S. A. Mahmoud, Effect of heavy metals accumulation on enzyme activity and histology in liver of some nile fish in egypt, Egypt. J. Aquat. Biol. Fish., 2005, 9, 203–219.

    Article  Google Scholar 

  40. V. Palanivelu, K. Vijayavel, S. E. Balasubramanian and M. P. Balasubramanian, Influence of insecticidal derivative (cartap hydrochloride) from the marine polychaete on certain enzyme systems of the fresh water fish oreochromismossambicus, J. Environ. Biol., 2005, 26, 191–195.

    CAS  PubMed  Google Scholar 

  41. A. G. M. Osman, A. M. Abd El Reheem, M. A. Moustafa, U. M. Mahmoud, K. Y. Abuel-Fadl and W. Kloas, In situ evaluation of the genotoxic potential of the river nile. I. Micronucleus and nuclear lesion tests of erythrocytes of oreochromis niloticus (linnaeus, 1758) and clarias gariepinus (burchell, 1822), Toxicol. Environ. Chem., 2011, 93, 1002–1017.

    Article  CAS  Google Scholar 

  42. N. Parveen and G. G. Shadab, Cytogenetic evaluation of cadmium chloride on Channa punctatus, J. Environ. Biol., 2012, 33, 663–666.

    CAS  PubMed  Google Scholar 

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Acknowledgements

The author gratefully acknowledges the support offered by the Faculty of Science, AI Azhar University (Assiut branch). The author is also grateful to the Associate Editor, Dr Patrick Neale, Research Scientist at Smithsonian EnvironmentaI Research Center, MaryIand, USA, for his cooperation and counseI throughout the writing of the manuscript and its revision. The Iast author is gratefuI for the continuous support from the AIexander von HumboIdt Foundation.

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Authors and Affiliations

  1. Department of Zoology, Faculty of Science, Assiut University (New Valley Branch), Egypt

    Ahmed Th. A. Ibrahim

  2. Lecturer of Fish biology and Ecology, Faculty of Fish and Fisheries Technology, Aswan University, Egypt

    Khaled Y. AbouelFadl

  3. Department of Zoology, Faculty of Science, Al-Azhar University (Assiut Branch), 71524, Assiut, Egypt

    Alaa G. M. Osman

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  1. Ahmed Th. A. Ibrahim
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  2. Khaled Y. AbouelFadl
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  3. Alaa G. M. Osman
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Correspondence to Ahmed Th. A. Ibrahim, Khaled Y. AbouelFadl or Alaa G. M. Osman.

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Ibrahim, A.T.A., AbouelFadl, K.Y. & Osman, A.G.M. Ultraviolet A-induced hematotoxic and genotoxic potential in Nile tilapia Oreochromis niloticus. Photochem Photobiol Sci 18, 1495–1502 (2019). https://doi.org/10.1039/c8pp00189h

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