Issue 22, 2018

Energy transfer chemiluminescence for ratiometric pH imaging

Abstract

Chemiluminescence imaging offers a low background and high sensitivity approach to imaging analytes in living cells and animals. Intensity-based measurements have been developed, but require careful consideration of kinetics, probe localization, and fluctuations in quantum yield, all of which complicate quantification. Here, we report a ratiometric strategy for quantitative chemiluminescence imaging of pH. The strategy relies on an energy transfer cascade of chemiluminescence emission from a spiroadamantane 1,2-dioxetane to a ratiometric pH indicator via fluorescent dyes in Enhancer solutions. Monitoring the pH-dependent changes in chemiluminescence emission at multiple wavelengths enables ratiometric imaging and quantification of pH independent from variations due to kinetics and probe concentration.

Graphical abstract: Energy transfer chemiluminescence for ratiometric pH imaging

Supplementary files

Article information

Article type
Paper
Submitted
25 Apr 2018
Accepted
17 May 2018
First published
17 May 2018

Org. Biomol. Chem., 2018,16, 4176-4182

Author version available

Energy transfer chemiluminescence for ratiometric pH imaging

W. An, R. P. Mason and A. R. Lippert, Org. Biomol. Chem., 2018, 16, 4176 DOI: 10.1039/C8OB00972D

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