Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GTBP1 with in situ product removal

Bingfeng Li; Xuejun He; Bo Fan; Jianlin Chu; Bingfang He

doi:10.1039/C7RA02367G

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Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GT_BP1 with in situ product removal†

Bingfeng Li,^ab Xuejun He,^b Bo Fan,^a Jianlin Chu^ac and Bingfang He

*^ac

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* Corresponding authors

^a College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, China
E-mail: bingfanghe@njtech.edu.cn
Fax: +86 25 5813 9902
Tel: +86 25 5813 9902

^b College of Biology and Environment, Nanjing Polytechnic Institute, China

^c School of Pharmaceutical Sciences, Nanjing Tech University, China

Abstract

A glycosyltransferase GT_BP1 from Bacillus pumilus BF1 was isolated. Efficient production of ponasterone A was achieved by the recombinant E. coli/gt_BP1 in a biphasic system with a molar yield of 92.7%. This in situ product removal provided the “driving force” for shifting the reaction equilibrium towards the synthesis of the product.

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Article information

DOI: https://doi.org/10.1039/C7RA02367G
Article type: Paper
Submitted: 25 Feb 2017
Accepted: 19 Apr 2017
First published: 26 Apr 2017
This article is Open Access

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RSC Adv., 2017,7, 23027-23029

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Efficient synthesis of ponasterone A by recombinant Escherichia coli harboring the glycosyltransferase GT_BP1 with in situ product removal

B. Li, X. He, B. Fan, J. Chu and B. He, RSC Adv., 2017, 7, 23027 DOI: 10.1039/C7RA02367G

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