Paclitaxel modified Fe3O4 loaded albumin nanoparticles as drug delivery vehicles by self-assembly
Abstract
In this paper, the preparation of Fe3O4/PTX/HSA nanoparticles with magnetic resonance imaging (MRI) and slow release functionality by a self-assembly method is presented. Firstly, hydrophobic Fe3O4 nanoparticles with an average size of 10 nm are synthesized by thermal decomposition of an iron–oleate complex and then modified and stabilized by hydrophobic paclitaxel (PTX). Lastly, Fe3O4/PTX nanoparticles with a 3 nm PTX shell on the Fe3O4 surface are loaded into human serum albumin (HSA) to form uniform Fe3O4/PTX/HSA nanoparticles by PTX–HSA interaction after breaking the disulfide bond and unfolding hydrophobic region of HSA. The novel PTX modified Fe3O4/PTX nanoparticles have good dispersity in ethanol and strong binding capacity with HSA, which can be homogenously dispersed in HSA matrices to form novel Fe3O4/PTX/HSA nanoparticles with a pie structure by the self-assembly method. The resulting Fe3O4/PTX/HSA nanoparticles with a high saturation magnetization value of 10.2 emu g−1, good T2 imaging functionality and excellent ability to cross the cell membrane have been demonstrated by magnetization curves, in vitro MRI and cellular uptake. Furthermore, the in vitro antitumor ability of the system has also been evaluated.
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