Issue 55, 2016

Genetically encoded optical activation of DNA recombination in human cells

Abstract

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

Graphical abstract: Genetically encoded optical activation of DNA recombination in human cells

Supplementary files

Article information

Article type
Communication
Submitted
10 May 2016
Accepted
28 May 2016
First published
09 Jun 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 8529-8532

Genetically encoded optical activation of DNA recombination in human cells

J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin and A. Deiters, Chem. Commun., 2016, 52, 8529 DOI: 10.1039/C6CC03934K

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