Issue 17, 2016
From the journal:

Organic & Biomolecular Chemistry

Synthesis of a peptide-universal nucleotide antigen: towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes†

Angela L. Perkins,a   Kevin L. Peterson,b   Thomas G. Beito,c   Karen S. Flatten,b   Scott H. Kaufmannb  and  Daniel A. Harki*a  

* Corresponding authors

a Department of Medicinal Chemistry, University of Minnesota, 2231 6th Street SE, Minneapolis, MN 55455, USA
E-mail: daharki@umn.edu
Tel: +1 612-625-8687

b Division of Oncology Research, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA

c Monoclonal Antibody Core, Mayo Clinic, 200 First St., S.W., Rochester, MN 55905, USA

Abstract

The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide 2, and four murine monoclonal antibodies raised against 2 that exhibit excellent specificity for recognition of 2 in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

Graphical abstract: Synthesis of a peptide-universal nucleotide antigen: towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

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Article information

DOI
https://doi.org/10.1039/C5OB02049B
Article type
Paper
Submitted
01 Oct 2015
Accepted
01 Apr 2016
First published
13 Apr 2016

Org. Biomol. Chem., 2016,14, 4103-4109

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Synthesis of a peptide-universal nucleotide antigen: towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

A. L. Perkins, K. L. Peterson, T. G. Beito, K. S. Flatten, S. H. Kaufmann and D. A. Harki, Org. Biomol. Chem., 2016, 14, 4103 DOI: 10.1039/C5OB02049B

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