Issue 8, 2015
From the journal:

Dalton Transactions

Convenient detection of metal–DNA, metal–RNA, and metal–protein adducts with a click-modified Pt(ii) complex†

Alan D. Moghaddam,‡a   Jonathan D. White,‡a   Rachael M. Cunningham,ab   Andrea N. Loes,ab   Michael M. Haleya  and  Victoria J. DeRose*ab  

* Corresponding authors

a Department of Chemistry & Biochemistry, University of Oregon, Eugene, OR 97403-1253, USA

b Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1253, USA
E-mail: derose@uoregon.edu
Tel: +1-541-346-3568

Abstract

cis-[Pt(2-azido-1,3-propanediamine)Cl2] is a reagent for high-yield post-treatment fluorescent labelling of Pt(II) biomolecular targets using click chemistry and exhibits a bias in conformational isomers in the context of duplex DNA. Pt–protein adducts are detected using BSA as a model. Following in vivo treatment, long-lived Pt–RNA adducts are detected on ribosomal RNA.

Graphical abstract: Convenient detection of metal–DNA, metal–RNA, and metal–protein adducts with a click-modified Pt(ii) complex

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4DT02649G
Article type
Communication
Submitted
29 Aug 2014
Accepted
08 Oct 2014
First published
08 Oct 2014
This article is Open Access
Creative Commons BY-NC license

Dalton Trans., 2015,44, 3536-3539

Author version available

Download author version (PDF)

Permissions

Request permissions

Convenient detection of metal–DNA, metal–RNA, and metal–protein adducts with a click-modified Pt(II) complex

A. D. Moghaddam, J. D. White, R. M. Cunningham, A. N. Loes, M. M. Haley and V. J. DeRose, Dalton Trans., 2015, 44, 3536 DOI: 10.1039/C4DT02649G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements