Issue 22, 2014
From the journal:

Chemical Communications

A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid†

Wei Wang,ab   Takanori Uzawa,ac   Naoya Tochio,d   Jumpei Hamatsu,e   Yoshinori Hirano,f   Seiichi Tada,a   Hisao Saneyoshi,a   Takanori Kigawa,d   Nobuhiro Hayashi,g   Yutaka Ito,e   Makoto Taiji,f   Toshiro Aigakib  and  Yoshihiro Ito*abc  

* Corresponding authors

a Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
E-mail: y-ito@riken.jp
Tel: +81-484675809

b Graduate School of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-Osawa, Tokyo 192-0397, Japan

c Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan

d NMR Pipeline Methodology Research Team, RIKEN Systems and Structural Biology Center, Yokohama, Kanagawa 230-0045, Japan

e Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Osawa, Tokyo 192-0397, Japan

f Laboratory for Computational Molecular Design, Computational Biology Research Core, RIKEN Quantitative Biology Center, 1-6-5 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan

g Department of Life Science, School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan

Abstract

A peptide that binds and emits fluorescence in response to conformational change in a target protein was developed by in vitro selection using tRNA carrying a fluorogenic amino acid. This technology could prove to be useful for the development of separation-free immunoassays and bio-imaging analyses.

Graphical abstract: A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

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Article information

DOI
https://doi.org/10.1039/C3CC47624C
Article type
Communication
Submitted
04 Oct 2013
Accepted
15 Nov 2013
First published
18 Nov 2013

Chem. Commun., 2014,50, 2962-2964

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A fluorogenic peptide probe developed by in vitro selection using tRNA carrying a fluorogenic amino acid

W. Wang, T. Uzawa, N. Tochio, J. Hamatsu, Y. Hirano, S. Tada, H. Saneyoshi, T. Kigawa, N. Hayashi, Y. Ito, M. Taiji, T. Aigaki and Y. Ito, Chem. Commun., 2014, 50, 2962 DOI: 10.1039/C3CC47624C

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