Issue 30, 2013
From the journal:

Chemical Communications

An ultrasensitive chemiluminescence turn-on assay for protease and inhibitor screening with a natural substrate†

Qingfeng Zhang,a   Wenying Li,ab   Jian Chen,a   Fangyuan Wang,ab   Yan Wang,ab   Yang Chenab  and  Cong Yu*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China
E-mail: congyu@ciac.jl.cn
Fax: +86 431-8526-2710

b University of the Chinese Academy of Sciences, Beijing 100049, P. R. China

Abstract

A highly sensitive and convenient chemiluminescence (CL) turn-on assay for protease without any label and synthesis has been developed. Cytochrome c was digested by a protease, and heme was released as a peptide–heme conjugate, which greatly enhanced the luminol–H2O2 CL reaction.

Graphical abstract: An ultrasensitive chemiluminescence turn-on assay for protease and inhibitor screening with a natural substrate

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Article information

DOI
https://doi.org/10.1039/C3CC40906F
Article type
Communication
Submitted
02 Feb 2013
Accepted
01 Mar 2013
First published
04 Mar 2013

Chem. Commun., 2013,49, 3137-3139

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An ultrasensitive chemiluminescence turn-on assay for protease and inhibitor screening with a natural substrate

Q. Zhang, W. Li, J. Chen, F. Wang, Y. Wang, Y. Chen and C. Yu, Chem. Commun., 2013, 49, 3137 DOI: 10.1039/C3CC40906F

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