Issue 18, 2013
From the journal:

Analytical Methods

Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses†

Sébastien Laulhé,a   Tyler E. Geers,a   Xue Shi,a   Xiang Zhanga  and  Michael H. Nantz*a  

* Corresponding authors

a University of Louisville, Department of Chemistry, 2320 South Brook Street, Louisville, Kentucky 40292, USA
E-mail: michael.nantz@louisville.edu
Tel: +1 502 852 8069

Abstract

We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement of isotopic ethyl carbenium ions serving as mass spectral tags in a zone of minimal interference (ZMI) at m/z 31–37. Sample aldehyde and ketone mixtures were reacted with isotopic 2-aminooxyethyl propionates to illustrate the method, which determined the relative abundance of the mixed compounds with an average 95% accuracy. ZMI reporter ion detection also enables chemoselective substrate profiling and absolute quantification, as demonstrated using a biologically derived sample.

Graphical abstract: Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses

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Article information

DOI
https://doi.org/10.1039/C3AY41124A
Article type
Paper
Submitted
08 Jul 2013
Accepted
09 Jul 2013
First published
25 Jul 2013

Anal. Methods, 2013,5, 4701-4706

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Electron ionization-induced release of coded isotopic reporter ions in an m/z zone of minimal interference for quantifiable, multiplexed GC-MS analyses

S. Laulhé, T. E. Geers, X. Shi, X. Zhang and M. H. Nantz, Anal. Methods, 2013, 5, 4701 DOI: 10.1039/C3AY41124A

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