Issue 15, 2013
From the journal:

Analyst

Nanoparticle embedded enzymes for improved lateral flow sensors†

Veli C. Özalp,*ab   Uğur S. Zeydanlı,c   Anita Lunding,d   Murat Kavruk,e   M. Tufan Öz,‡e   Füsun Eyidoğan,f   Lars F. Olsend  and  Hüseyin A. Öktemeb  

* Corresponding authors

a School of Medicine, Istanbul Kemerburgaz University, Istanbul, Turkey
E-mail: cengiz.ozalp@kemerburgaz.edu.tr

b Nanobiz Ltd, METU Technopolis, Galium Block, 2. Floor, no:18, Ankara, Turkey

c Nature Conservation Centre, Aşağı Öveçler Mah. 1065. Cad. 1293 Sok. No. 9/32, 06460 Ankara, Turkey

d Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark

e Department of Biology, Nanobiotechnology R&D Lab, Middle East Technical University, Ankara, Turkey

f Başkent University, Faculty of Education, Ankara, Turkey

Abstract

In this study, combining the nanoparticle embedded sensors with lateral flow assays, a novel strategy for ensuring the quality of signalling in lateral flow assays (LFAs) was developed. A LFA for reactive oxygen species (ROS) is reported that is based on horse radish peroxidase (HRP) which is co-entrapped with Texas Red dextran inside porous polyacrylamide nanoparticles. In this system, enzymes are protected in the porous matrix of polyacrylamide which freely allows the diffusion of the analyte. The sensor is rapid and sensitive for quantification of hydrogen peroxide concentrations. A test solution of hydrogen peroxides was quantified with this novel LFA-ROS sensor to obtain a linear range between 1 and 25 μM. Nanoparticle embedding of enzymes is proposed here as a general strategy for developing enzyme-based lateral flow assays, eliminating adverse effects associated with biological samples.

Graphical abstract: Nanoparticle embedded enzymes for improved lateral flow sensors

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Article information

DOI
https://doi.org/10.1039/C3AN00733B
Article type
Communication
Submitted
12 Apr 2013
Accepted
10 May 2013
First published
13 May 2013

Analyst, 2013,138, 4255-4259

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Nanoparticle embedded enzymes for improved lateral flow sensors

V. C. Özalp, U. S. Zeydanlı, A. Lunding, M. Kavruk, M. T. Öz, F. Eyidoğan, L. F. Olsen and H. A. Öktem, Analyst, 2013, 138, 4255 DOI: 10.1039/C3AN00733B

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