Issue 9, 2013
From the journal:

Analyst

Simultaneous multi-mycotoxin determination in nutmeg by ultrasound-assisted solid–liquid extraction and immunoaffinity column clean-up coupled with liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection

Wei-Jun Kong,†a   Shu-Yu Liu,†a   Feng Qiu,a   Xiao-He Xiaob  and  Mei-Hua Yang*ac  

* Corresponding authors

a Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China
E-mail: yangmeihua15@hotmail.com
Tel: +86 10 57833277

b China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China

c Hainan Branch Institute of Medicinal Plant (Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine), Chinese Academy of Medical Sciences & Peking Union Medical College, Wanning 571533, China

Abstract

A simple and sensitive analytical method based on ultrasound-assisted solid–liquid extraction and immunoaffinity column clean-up coupled with high performance liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection (USLE-IAC-HPLC-PCD-FLD) has been developed for simultaneous multi-mycotoxin determination of aflatoxins B1, B2, G1, G2 (AFB1, AFB2, AFG1, AFG2) and ochratoxin A (OTA) in 13 edible and medicinal nutmeg samples marketed in China. AFs and OTA were extracted from nutmeg samples by ultrasonication using a methanol : water (80 : 20, v/v) solution, followed by an IAC clean-up step. Different USL extraction conditions, pre-processing ways for nutmeg sample and clean-up columns for mycotoxins, as well as HPLC-PCD-FLD parameters (mobile phase, column temperature, elution procedure, excitation and emission wavelengths) were optimized. This method, which was appraised for analyzing nutmeg samples, showed satisfactory results with reference to limits of detection (LODs) (from 0.02 to 0.25 μg kg−1), limits of quantification (LOQs) (from 0.06 to 0.8 μg kg−1), linear ranges (up to 30 ng mL−1 for AFB1, AFG1 and OTA and 9 ng mL−1 for AFB2 and AFG2), intra- and inter-day variability (all <2%) and average recoveries (from 79.6 to 90.8% for AFs and from 93.6 to 97.3% for OTA, respectively). The results of the application of developed method in nutmeg samples have elucidated that four samples were detected with contamination of AFs and one with OTA. AFB1 was the most frequently found mycotoxin in 30.8% of nutmeg samples at contamination levels of 0.73–16.31 μg kg−1. At least two different mycotoxins were co-occurred in three samples, and three AFs were simultaneously detected in one sample.

Graphical abstract: Simultaneous multi-mycotoxin determination in nutmeg by ultrasound-assisted solid–liquid extraction and immunoaffinity column clean-up coupled with liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C3AN00059A
Article type
Paper
Submitted
09 Jan 2013
Accepted
31 Jan 2013
First published
01 Feb 2013

Analyst, 2013,138, 2729-2739

Permissions

Request permissions

Simultaneous multi-mycotoxin determination in nutmeg by ultrasound-assisted solid–liquid extraction and immunoaffinity column clean-up coupled with liquid chromatography and on-line post-column photochemical derivatization-fluorescence detection

W. Kong, S. Liu, F. Qiu, X. Xiao and M. Yang, Analyst, 2013, 138, 2729 DOI: 10.1039/C3AN00059A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements