Issue 20, 2012
From the journal:

Lab on a Chip

Enantioselective analysis of melagatran via an LSPR biosensor integrated with a microfluidic chip†

Longhua Guo,*a   Yuechun Yin,a   Rong Huang,a   Bin Qiu,a   Zhenyu Lin,a   Huang-Hao Yang,a   Jianrong Libc  and  Guonan Chen*a  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, China
E-mail: guolh@fzu.edu.cn, gnchen@fzu.edu.cn

b Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China

c Food Safety Key Laboratory of Liaoning Province, Bohai University, Jinzhou, China

Abstract

The impact of chiral compounds on pharmacological and biological processes is well known. With the increasing need for enantiomerically pure compounds, effective strategies for enantioseparation and chiral discrimination are in great demand. Herein we report a simple but efficient approach for the enantioselective determination of chiral compounds based on a localized surface plasmon resonance (LSPR) biosensor integrated with a microfluidic chip. A glass microfluidic chip with an effective volume of ∼0.75 μL was fabricated for this application. Gold nanorods (AuNRs) with an aspect ratio of ∼2.6 were self-assembled onto the surface of the inner wall of the chip to serve as LSPR transducers, which would translate the analyte binding events into quantitative concentration information. Human α-thrombin was immobilized onto the AuNR surface for enantioselective sensing of the enantiomers of melagatran. The proposed sensor was found to be highly selective for RS-melagatran, while the binding of its enantiomer, SR-melagatran, to the sensor was inactive. Under optimal conditions, the limit of detection of this sensor for RS-melagatran was found to be 0.9 nM, whereas the presence of 10 000-fold amounts of SR-melagatran did not interfere with the detection. To the best of our knowledge, this is the first demonstration of an LSPR-based enantioselective biosensor.

Graphical abstract: Enantioselective analysis of melagatran via an LSPR biosensor integrated with a microfluidic chip

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Article information

DOI
https://doi.org/10.1039/C2LC40388A
Article type
Paper
Submitted
21 Apr 2012
Accepted
15 Jun 2012
First published
19 Jun 2012

Lab Chip, 2012,12, 3901-3906

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Enantioselective analysis of melagatran via an LSPR biosensor integrated with a microfluidic chip

L. Guo, Y. Yin, R. Huang, B. Qiu, Z. Lin, H. Yang, J. Li and G. Chen, Lab Chip, 2012, 12, 3901 DOI: 10.1039/C2LC40388A

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