Issue 30, 2012
From the journal:

Journal of Materials Chemistry

Preparation of aminosilane–alginate hybrid microcapsules and their use for enzyme encapsulation

Fumio Kurayama,*a   Satoru Suzuki,b   Newaz Mohammed Bahadur,cd   Takeshi Furusawa,b   Hajime Ota,b   Masahide Satob  and  Noboru Suzukiabc  

* Corresponding authors

a Center for Optical Research & Education, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan
E-mail: fkurayama@cc.utsunomiya-u.ac.jp
Fax: +81-28-689-6163
Tel: +81-28-689-6163

b Department of Advanced Interdisciplinary Sciences, Faculty of Engineering, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan

c Venture Business Laboratory, Utsunomiya University, 7-1-2 Yoto, Utsunomiya, Tochigi, Japan

d Department of Applied Chemistry and Chemical Engineering, Noakhali Science and Technology University, Noakhali-3802, Bangladesh

Abstract

Organic–inorganic hybrid microcapsules (AP-capsules) were fabricated by sequential preparation of calcium alginate microcapsules (Alg-capsules), followed by the addition of the Alg-capsules to an aqueous solution of 3-aminopropyltriethoxysilane (APTES) in mild conditions without the use of any catalyst. Results showed that the shell thickness of AP-capsules was found to vary with time during the APTES incorporation onto the Alg-capsules, suggesting that the shell thickness was dependent on the amount of CaCl2 contained initially in the core solution as well as on the reaction time. A model enzyme, formate dehydrogenase (FDH), was successfully encapsulated in AP-capsules, and maximum efficiency of encapsulation was found with the capsule size of 1 mm diameter. The AP-capsules were reused efficiently for 10 cycles without loss of FDH activity. The approach developed in this study could evolve as a generic platform of enzyme immobilization for biotechnology applications using core–shell microcapsule technology.

Graphical abstract: Preparation of aminosilane–alginate hybrid microcapsules and their use for enzyme encapsulation

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Article information

DOI
https://doi.org/10.1039/C2JM31792C
Article type
Paper
Submitted
22 Mar 2012
Accepted
31 May 2012
First published
01 Jun 2012

J. Mater. Chem., 2012,22, 15405-15411

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Preparation of aminosilane–alginate hybrid microcapsules and their use for enzyme encapsulation

F. Kurayama, S. Suzuki, N. M. Bahadur, T. Furusawa, H. Ota, M. Sato and N. Suzuki, J. Mater. Chem., 2012, 22, 15405 DOI: 10.1039/C2JM31792C

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