Issue 5, 2013
From the journal:

Analytical Methods

An eco-friendly hydrophilic interaction HPLC method for the determination of renal function biomarkers, creatinine and uric acid, in human fluids

Si Zhou,a   Ruixiao Zuo,b   Zhuo Zhu,a   Di Wu,a   Kruti Vasa,a   Yiwei Dengc  and  Yuegang Zuo*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, MA 02747, USA
E-mail: yzuo@umassd.edu
Fax: +1 508-999-9167
Tel: +1 508-999-8959

b Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA

c Department of Natural Sciences, University of Michigan, Dearborn, MI 48128, USA

Abstract

An accurate, robust and environmentally friendly hydrophilic interaction liquid chromatographic (HILIC) method was developed for the determination of renal function biomarkers, creatinine and uric acid, in human urine and tear samples. The sample preparation was simple and fast with the steps of protein precipitation with 2.0 mL of 50% acetonitrile, centrifugation, filtration and dilution. A weak polar cyano HILIC stationary phase with a highly aqueous mobile phase was chosen for the separation to minimize the consumption of harmful organic solvents. This developed HILIC method consumed less than 0.25 mL of acetonitrile per sample separation. Quantitation was carried out by an internal standard method. A good linearity of response in the concentration range of 0.20 to 10.0 μg mL−1 creatinine or uric acid with a coefficient R2 = 0.999 for quantitative analysis was achieved. The detection limits for creatinine and uric acid were 0.04 and 0.06 μg mL−1, respectively. This method has been successfully applied for the determination of creatinine and uric acid in human urine and tear samples.

Graphical abstract: An eco-friendly hydrophilic interaction HPLC method for the determination of renal function biomarkers, creatinine and uric acid, in human fluids

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Article information

DOI
https://doi.org/10.1039/C2AY26362A
Article type
Paper
Submitted
08 Nov 2012
Accepted
04 Jan 2013
First published
08 Jan 2013

Anal. Methods, 2013,5, 1307-1311

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An eco-friendly hydrophilic interaction HPLC method for the determination of renal function biomarkers, creatinine and uric acid, in human fluids

S. Zhou, R. Zuo, Z. Zhu, D. Wu, K. Vasa, Y. Deng and Y. Zuo, Anal. Methods, 2013, 5, 1307 DOI: 10.1039/C2AY26362A

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