Issue 11, 2011
From the journal:

Molecular BioSystems

Proteomic analysis of the Mexican lime tree response to “Candidatus Phytoplasma aurantifolia” infection†

Farzan Taheri,ab   Ghorbanali Nematzadeh,b   Maryam Ghayeb Zamharir,c   Mojtaba Khayam Nekouei,d   Mohammadreza Naghavi,e   Mohsen Mardi*d  and  Ghasem Hosseini Salekdeh*af  

* Corresponding authors

a Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, P.O. Box 31535-1897, Karaj, Tehran, Iran
E-mail: h_salekdeh@abrii.ac.ir
Fax: +98-261-2704539

b Department of Agronomy, Plant Breeding and Biotechnology, College of Agricultural Sciences, University of Agricultural Sciences and Natural Resources of Sari, Iran

c Laboratory of Prokaryotes, Department of Plant Disease, Iranian Research Institute of Plant Protection, Tehran, Iran

d Department of Genomics, Agricultural Biotechnology Research Institute of Iran, P.O. Box 31535-1897, Karaj, Tehran, Iran
E-mail: Mardi@abrii.ac.ir
Fax: +98-261-2704539

e Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

f Department of Molecular Systems Biology, Royan Institute, Tehran, Iran

Abstract

“Candidatus Phytoplasma aurantifolia” is the causative agent of witches' broom disease in the Mexican lime tree (Citrus aurantifolia L.), and is responsible for major tree losses in Southern Iran and Oman. The pathogen is strictly biotrophic, and, therefore, completely dependent on living host cells for its survival. The molecular basis of compatibility and disease development in this system is poorly understood. We applied a proteomics approach to analyse gene expression in Mexican limes infected with “Ca. Phytoplasma aurantifolia”. Leaf samples were collected from healthy and infected plants and were analysed using 2-DE coupled with MS. Among 800 leaf proteins that were detected reproducibly in eight biological replicates of healthy and eight biological replicates of infected plants, 55 showed a significant response to the disease. MS resulted in identification of 39 regulated proteins, which included proteins that were involved in oxidative stress defence, photosynthesis, metabolism, and the stress response. Our results provide the first proteomic view of the molecular basis of the infection process and identify genes that could help inhibit the effects of the pathogen.

Graphical abstract: Proteomic analysis of the Mexican lime tree response to “Candidatus Phytoplasma aurantifolia” infection

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1MB05268C
Article type
Paper
Submitted
28 Jun 2011
Accepted
27 Jul 2011
First published
19 Aug 2011

Mol. BioSyst., 2011,7, 3028-3035

Permissions

Request permissions

Proteomic analysis of the Mexican lime tree response to “Candidatus Phytoplasma aurantifolia” infection

F. Taheri, G. Nematzadeh, M. G. Zamharir, M. K. Nekouei, M. Naghavi, M. Mardi and G. H. Salekdeh, Mol. BioSyst., 2011, 7, 3028 DOI: 10.1039/C1MB05268C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements