Issue 14, 2011
From the journal:

Analyst

Fluorescence signal transduction mechanism for immunoassay based on zinc ion release from ZnS nanocrystals†

Chad L. Cowles,a   Xiaoshan Zhu*a  and  Nelson G. Publicovera  

* Corresponding authors

a Department of Electrical and Biomedical Engineering University of Nevada Reno Reno, Nevada, USA
E-mail: xzhu@unr.edu
Fax: +1-775-784-6627

Abstract

In this work, a fluorescence signal transduction mechanism based on cation release from ZnS nanocrystals was developed for sandwich immunoassay. In this mechanism, ZnS nanocrystals as labels in immunoassay are dissolved by acid to release zinc ions. After pH adjustment of the dissolving solution using a basic solution, zinc-ion sensitive fluorescence indicator Fluozin-3 is added to bind with the released zinc ions for sensitive fluorescence measurement. Using mouse IgG as a model analyte, the immunoassay adopting this signal transduction mechanism demonstrates a low detection limit around 1 pM and a detection range with two orders of magnitude (1 pM to 0.5 nM).

Graphical abstract: Fluorescence signal transduction mechanism for immunoassay based on zinc ion release from ZnS nanocrystals

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1AN15274B
Article type
Paper
Submitted
03 Apr 2011
Accepted
12 May 2011
First published
06 Jun 2011

Analyst, 2011,136, 2975-2980

Permissions

Request permissions

Fluorescence signal transduction mechanism for immunoassay based on zinc ion release from ZnS nanocrystals

C. L. Cowles, X. Zhu and N. G. Publicover, Analyst, 2011, 136, 2975 DOI: 10.1039/C1AN15274B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements