Issue 18, 2010
From the journal:

Soft Matter

Programming the kinetics and extent of colloidal disassembly using a DNA trigger†

Christopher Kirby Tisona  and  Valeria Tohver Milam*abc  

* Corresponding authors

a School of Materials Science & Engineering, Georgia Institute of Technology, 711 Ferst Dr NW, Atlanta, GA
E-mail: valeria.milam@mse.gatech.edu
Fax: +404 385 3734
Tel: +404 894 2845

b Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 315 Ferst Dr. NW, Atlanta, GA

c Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 313 Ferst Dr. NW, Atlanta, GA

Abstract

We present experimental studies of the effects of DNA sequence length and concentration on the kinetics of competitive hybridization events in colloidal suspensions. We quantify both the number of displaced soluble target strands and the extent of disassembly of DNA-linked colloidal micelles or colloidal satellites using flow cytometry. The kinetics of competitive displacement of soluble targets are on the order of minutes whereas disassembly of the colloidal micelles requires several hours and even days. By fitting the data to a first-order equation, we found that the observed replacement rate for the soluble target system depends on target base length differences as well as the concentration of the competitive strands. Our studies illustrate that DNA can be employed as a programmable, isothermal disassembly tool for DNA-linked colloidal particles.

Graphical abstract: Programming the kinetics and extent of colloidal disassembly using a DNA trigger

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Article information

DOI
https://doi.org/10.1039/C0SM00222D
Article type
Paper
Submitted
09 Apr 2010
Accepted
26 May 2010
First published
30 Jul 2010

Soft Matter, 2010,6, 4446-4453

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Programming the kinetics and extent of colloidal disassembly using a DNA trigger

C. K. Tison and V. T. Milam, Soft Matter, 2010, 6, 4446 DOI: 10.1039/C0SM00222D

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