Issue 10, 2010
From the journal:

Journal of Analytical Atomic Spectrometry

Comparison of ICP-MS and photometric detection of an immunoassay for the determination of ochratoxin A in wine

Charlotte Giesen,ab   Norbert Jakubowski,b   Ulrich Panneab  and  Michael G. Weller*b  

* Corresponding authors

a Humboldt-Universitaet zu Berlin, Department of Chemistry, Brook-Taylor-Strasse 2, Berlin, Germany

b BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Strasse 11, Berlin, Germany
E-mail: michael.weller@bam.de

Abstract

An immunoassay was developed for ochratoxin A (OTA) detection in wine and two completely different detection methods were compared: ICP-MS and photometry. For labelling, we applied secondary antibodies conjugated with gold nanoparticles for ICP-MS and with horseradish peroxidase for photometric detection, respectively. Detection limits of the assay were determined as 0.003 µg L−1 for both detection methods. OTA in wine was determined below the EU limit value of 2 µg kg−1. The measurement range was between 0.01 and 1 µg L−1 for ICP-MS and photometric detection. Assay precision detected by ICP-MS and photometry were similar showing that precision of the detection method has only a minor effect on total assay precision. By using BSA as a buffer additive to minimize nonspecific binding, we could also confirm that OTA strongly binds to BSA. In a comparison study of several additives, casein was found to be preferable.

Graphical abstract: Comparison of ICP-MS and photometric detection of an immunoassay for the determination of ochratoxin A in wine

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Article information

DOI
https://doi.org/10.1039/C0JA00009D
Article type
Communication
Submitted
22 Apr 2010
Accepted
16 Jul 2010
First published
13 Aug 2010

J. Anal. At. Spectrom., 2010,25, 1567-1572

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Comparison of ICP-MS and photometric detection of an immunoassay for the determination of ochratoxin A in wine

C. Giesen, N. Jakubowski, U. Panne and M. G. Weller, J. Anal. At. Spectrom., 2010, 25, 1567 DOI: 10.1039/C0JA00009D

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