Issue 3, 2011
From the journal:

Analytical Methods

A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes†

Alsu I. Zamaleeva,‡a   Ilziya R. Sharipova,‡a   Rezeda V. Shamagsumova,b   Alexey N. Ivanov,b   Gennady A. Evtugyn,b   Dilara G. Ishmuchametovaa  and  Rawil F. Fakhrullin*a  

* Corresponding authors

a Biomaterials and Nanomaterials Group, Department of Biochemistry, Kazan (Idel buye/Volga region) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, RF
E-mail: kazanbio@gmail.com
Tel: +78432337833

b Department of Analytical Chemistry, Kazan (Idel buye/Volga region) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan, RF

Abstract

We report the fabrication of an amperometric whole-cell herbicide biosensor based on magnetic retention of living cells functionalised with magnetic nanoparticles (MNPs) on the surface of a screen-printed electrode. We demonstrate that Chlorella pyrenoidosa microalgae cells coated with biocompatible MNPs and retained on the electrode with a permanent magnet act as a sensing element for the fast detection of herbicides. The magnetic functionalisation does not affect the viability and photosynthesis activity-mediated triazine herbicide recognition in microalgae. The current of ferricyanide ion was recorded during alternating illumination periods and biosensor fabricated was used to detect atrazine (from 0.9 to 74 µM) and propazine (from 0.6 to 120 µM) (the limits of detection 0.7 and 0.4 µM, respectively). We believe that the methodology presented here can be widely used in fabrication of a number of whole cell biosensors since it allows for efficient and reversible cells immobilisation and does not affect the cellular metabolism.

Graphical abstract: A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes

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Article information

DOI
https://doi.org/10.1039/C0AY00627K
Article type
Communication
Submitted
18 Oct 2010
Accepted
02 Jan 2011
First published
24 Jan 2011

Anal. Methods, 2011,3, 509-513

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A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes

A. I. Zamaleeva, I. R. Sharipova, R. V. Shamagsumova, A. N. Ivanov, G. A. Evtugyn, D. G. Ishmuchametova and R. F. Fakhrullin, Anal. Methods, 2011, 3, 509 DOI: 10.1039/C0AY00627K

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