Issue 7, 2010
From the journal:

Analytical Methods

Cell-based impedance spectroscopy for probing inhibitory effects of steroids and ergostane/lanosta-related compounds†

Keith B. Male,a   Stephan M. Crowley,b   Stuart G. Collins,b   Yew-Min Tzengc  and  John H. T. Luong*ab  

* Corresponding authors

a Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada
E-mail: john.luong@cnrc-nrc.gc.ca

b Department of Chemistry, University College Cork, Cork, Ireland

c Institute of Biotechnology, Chaoyang University of Technology, Wufeng, Taiwan, Republic of China

Abstract

Electric cell–substrate impedance sensing (ECIS) was used for probing inhibitory effects on Spodoptera frugiperda Sf9 insect cells exposed to five synthesized steroid compounds. The results were compared to the levels obtained using three ergostane-related steroids and five lanosta-related triterpenes purified from the fruiting bodies of Antrodia camphorata. The half-inhibition concentration (ECIS50), the level of 50% inhibition of the resistance response, was determined from the response function to establish inhibitory effects of the different compounds. Significant effects on inhibition as probed by impedance spectroscopy were noticed because of slight changes in chemical structure. Only two of the synthesized compounds, 24(R,S)-5α-lanost-8-ene-3,24,25-triol and 5α-lanost-8-ene-3,7,11-trione, showed inhibitory effects which were much less significant compared to the A. camphorata steroids. The ECIS50 values were 150–250 µM, similar to the value for the least inhibitory lanosta-related triterpene. This noninvasive measurement in combination with Sf9 insect cells has been proven as a simple and reliable tool for screening inhibition/cytotoxicity and designing steroid-related compounds.

Graphical abstract: Cell-based impedance spectroscopy for probing inhibitory effects of steroids and ergostane/lanosta-related compounds

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Article information

DOI
https://doi.org/10.1039/C0AY00105H
Article type
Paper
Submitted
11 Feb 2010
Accepted
25 Apr 2010
First published
18 May 2010

Anal. Methods, 2010,2, 870-877

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Cell-based impedance spectroscopy for probing inhibitory effects of steroids and ergostane/lanosta-related compounds

K. B. Male, S. M. Crowley, S. G. Collins, Y. Tzeng and J. H. T. Luong, Anal. Methods, 2010, 2, 870 DOI: 10.1039/C0AY00105H

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