Issue 7, 2010
From the journal:

Analyst

Microfabricated FSCV-compatible microelectrode array for real-time monitoring of heterogeneous dopamine release

Matthew K. Zachek,ab   Jinwoo Park,c   Pavel Takmakov,c   R. Mark Wightmanc  and  Gregory S. McCarty*ab  

* Corresponding authors

a Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

b Joint Department of Biomedical Engineering, NC State University, BME Department, 2147 Burlington Labs, 2500 Stinson Drive, Campus Box 7115, Raleigh, NC, USA
E-mail: gsmccart@ncsu.edu
Fax: +1 919-513-3814
Tel: +1 919-513-7933

c Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

Abstract

Fast scan cyclic voltammetry (FSCV) has been used previously to detect neurotransmitter release and reuptake in vivo. An advantage that FSCV has over other electrochemical techniques is its ability to distinguish neurotransmitters of interest (i.e. monoamines) from their metabolites using their respective characteristic cyclic voltammograms. While much has been learned with this technique, it has generally only been used in a single working electrode arrangement. Additionally, traditional electrode fabrication techniques tend to be difficult and somewhat irreproducible. Described in this report is a fabrication method for a FSCV compatible microelectrode array (FSCV-MEA) that is capable of functioning in vivo. The microfabrication techniques employed here allow for better reproducibility than traditional fabrication methods of carbon fiber microelectrodes, and enable batch fabrication of electrode arrays. The reproducibility and electrochemical qualities of the probes were assessed along with crosstalk in vitro. Heterogeneous release of electrically evoked dopamine was observed in real-time in the striatum of an anesthetized rat using the FSCV-MEA. The heterogeneous effects of pharmacology on the striatum were also observed and shown to be consistent across multiple animals.

Graphical abstract: Microfabricated FSCV-compatible microelectrode array for real-time monitoring of heterogeneous dopamine release

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Article information

DOI
https://doi.org/10.1039/C0AN00114G
Article type
Paper
Submitted
26 Feb 2010
Accepted
23 Apr 2010
First published
13 May 2010

Analyst, 2010,135, 1556-1563

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Microfabricated FSCV-compatible microelectrode array for real-time monitoring of heterogeneous dopamine release

M. K. Zachek, J. Park, P. Takmakov, R. M. Wightman and G. S. McCarty, Analyst, 2010, 135, 1556 DOI: 10.1039/C0AN00114G

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