Issue 13, 2010
From the journal:

Lab on a Chip

Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling†

Kirsty J. Shaw,a   Peter T. Docker,a   John V. Yelland,b   Charlotte E. Dyer,c   John Greenman,c   Gillian M. Greenwaya  and  Stephen J. Haswell*a  

* Corresponding authors

a Department of Chemistry, University of Hull, Cottingham Road, Hull, UK
E-mail: s.j.haswell@hull.ac.uk
Fax: +44 (0)1482 466410
Tel: +44 (0)1482 465475

b Exxel Amplifiers Ltd., 4 Pinfold Lane, Norfolk, UK

c Centre for Biomedical Research, University of Hull, Cottingham Road, Hull, UK

Abstract

A microwave heating system is described for performing polymerase chain reaction (PCR) in a microfluidic device. The heating system, in combination with air impingement cooling, provided rapid thermal cycling with heating and cooling rates of up to 65 °C s−1 and minimal over- or under-shoot (±0.1 °C) when reaching target temperatures. In addition, once the required temperature was reached it could be maintained with an accuracy of ±0.1 °C. To demonstrate the functionality of the system, PCR was successfully performed for the amplification of the Amelogenin locus using heating rates and quantities an order of magnitude faster and smaller than current commercial instruments.

Graphical abstract: Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling

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Article information

DOI
https://doi.org/10.1039/C000357N
Article type
Technical Note
Submitted
06 Jan 2010
Accepted
06 Apr 2010
First published
23 Apr 2010

Lab Chip, 2010,10, 1725-1728

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Rapid PCR amplification using a microfluidic device with integrated microwave heating and air impingement cooling

K. J. Shaw, P. T. Docker, J. V. Yelland, C. E. Dyer, J. Greenman, G. M. Greenway and S. J. Haswell, Lab Chip, 2010, 10, 1725 DOI: 10.1039/C000357N

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