Issue 6, 2009
From the journal:

Analyst

Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy†

T. J. Harvey,a   E. Gazi,b   A. Henderson,a   R. D. Snook,a   N. W. Clarke,b   M. Brownb  and  P. Gardner*a  

* Corresponding authors

a School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK
E-mail: peter.gardner@manchester.ac.uk
Fax: +44 (0)161 306 5201
Tel: +44 (0)161 306 4463

b Genito-Urinary Cancer Research Group, School of Cancer and Imaging Studies, Paterson Institute for Cancer Research, University of Manchester, Christie Hospital NHS Trust, Manchester, UK

Abstract

In this study we obtained Fourier transform infrared (FTIR) spectra of fixed prostate cell lines of differing types as well as the primary epithelial cells from benign prostatic hyperplasia (BPH). Results showed that by using multivariate chemometric analysis it was possible to discriminate and classify these cell lines, which gave rise to sensitivity and specificity values of >94% and >98%, respectively. Following on from these results the possible influences of different factors on the discrimination and classification of the prostate cell lines were examined. Firstly, the effect of using different growth media during cell culturing was investigated, with results indicating that this did not influence chemometric discrimination. Secondly, differences in the nucleus-to-cytoplasm (N/C) ratio were examined, and it was concluded that this factor was not the main reason for the discrimination and classification of the prostate cancer (CaP) cell lines. In conclusion, given the fact that neither growth media nor N/C ratio could totally explain the classification it is likely that actual biochemical differences between the cell lines is the major contributing factor.

Graphical abstract: Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy

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Article information

DOI
https://doi.org/10.1039/B903249E
Article type
Paper
Submitted
16 Feb 2009
Accepted
07 Apr 2009
First published
17 Apr 2009

Analyst, 2009,134, 1083-1091

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Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy

T. J. Harvey, E. Gazi, A. Henderson, R. D. Snook, N. W. Clarke, M. Brown and P. Gardner, Analyst, 2009, 134, 1083 DOI: 10.1039/B903249E

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