Issue 9, 2009
From the journal:

Lab on a Chip

Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability†

Choong Kim,ab   Kang Sun Lee,ac   Young Eun Kim,a   Kyu-Jung Lee,b   Soo Hyun Lee,a   Tae Song Kima  and  Ji Yoon Kang*a  

* Corresponding authors

a Nano-Bio Research Center, Korea Institute of Science and Technology, Seoul, Korea
E-mail: jykang@kist.re.kr
Tel: +82-2-958-6747

b School of Mechanical Engineering, Korea University, Korea

c Department of Electrical Engineering, The State University of New York, Buffalo, USA

Abstract

This paper describes a microfluidic device for the microencapsulation of cells in alginate beads to enhance cell viability. The alginate droplet including cells was gelified with calcified oleic acid, using two-phase microfluidics. The on-chip gelation had generated monodisperse spherical alginate beads, which could not be readily obtained via conventional external gelation in a calcium chloride bath. However, the prolonged exposure of encapsulated cells to the toxic oil phase caused serious damage to the cells. Therefore, we proposed the formulation of a rapid oil-exchange chip which transforms the toxic oleic acid to harmless mineral oil. The flushing out of oleic acid after the gelation of alginate beads effected a dramatic increase in the viability of P19 embryonic carcinoma cells, up to 90%. The experimental results demonstrated that the cell viability was proportional to the flow rate of squeezing mineral oil.

Graphical abstract: Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability

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Article information

DOI
https://doi.org/10.1039/B819044E
Article type
Technical Note
Submitted
28 Oct 2008
Accepted
26 Jan 2009
First published
18 Feb 2009

Lab Chip, 2009,9, 1294-1297

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Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability

C. Kim, K. S. Lee, Y. E. Kim, K. Lee, S. H. Lee, T. S. Kim and J. Y. Kang, Lab Chip, 2009, 9, 1294 DOI: 10.1039/B819044E

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