Issue 9, 2008
From the journal:

Analyst

An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity†‡

Haode Chen,a   Xiaolei Zuo,b   Shao Su,b   Zhuzhao Tang,a   Aibo Wu,a   Shiping Song,b   Dabing Zhang*a  and  Chunhai Fan*b  

* Corresponding authors

a College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
E-mail: zhangdb@sjtu.edu.cn
Fax: +86-21-5955-6902; +86-21-3420-4869

b Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
E-mail: fchh@sinap.ac.cn

Abstract

There has been urgent demand for rapid, sensitive and cost-effective pesticide assay technologies due to the global attention of environmental and food-safety problems. Acetycholinesterase (AChE)-based electrochemical sensors have attracted significant interest toward this goal. In this contribution, we introduced multiwalled carbon nanotubes (MWNTs) into our sensor design, where they played dual enhancement roles; first is that MWNTs loaded on glassy carbon (GC) electrodes significantly increase surface areas, facilitating the electrochemical polymerization of prussian blue (PB), a redox mediator for the electrochemical oxidation of the enzymatic product, thiocholine (TCh). Second, MWNTs enhance the enzymatic activity of AChE, as manifested by the decreased Michaelis–Menten constant (Km). As a result of these two important enhancement factors offered by MWNTs, our electrochemical pesticide sensor exhibited rapid response and high sensitivity toward the detection of a series of pesticides. Moreover, we demonstrated that this sensor was stable, reproducible and selective enough for detection in real samples.

Graphical abstract: An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity

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Article information

DOI
https://doi.org/10.1039/B805334K
Article type
Paper
Submitted
31 Mar 2008
Accepted
19 Jun 2008
First published
21 Jul 2008

Analyst, 2008,133, 1182-1186

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An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity

H. Chen, X. Zuo, S. Su, Z. Tang, A. Wu, S. Song, D. Zhang and C. Fan, Analyst, 2008, 133, 1182 DOI: 10.1039/B805334K

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