Issue 9, 2008
From the journal:

Analyst

Surface plasmon resonance imaging of limited glycoprotein samples†

Wei Liu,ac   Yi Chen*a  and  Mingdi Yanb  

* Corresponding authors

a Beijing National Laboratory of Molecular Science; Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry Chinese Academy of Sciences, Beijing, P. R. China
E-mail: chenyi@iccas.ac.cn

b Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon, USA

c Graduate School Chinese Academy of Sciences, Beijing, P. R. China

Abstract

A surface plasmon resonance imaging method has been developed for high throughput recognition and determination of low level glycoproteins with limited sample volume at least down to 50 nL. Chicken ovalbumin and immunoglobulin G were chosen as model compounds while bovine serum albumin and lysozyme were used as control. Each protein, at a concentration of 0.0080–1.0 mg mL−1, was printed on one gold sensing film, and the films were simultaneously reacted with a probe solution and viewed using a laboratory-built surface plasmon resonance imaging system. The imaging signals were dependent on the concentration and the type of analyte, with a limit of detection down to at least 0.5 ng. The glycoproteins dotted at either 1.0 mg mL−1 or 0.010 mg mL−1 were easily differentiated from the non-glycoproteins by reaction with 200 nM concanavalin A (con A), giving a limit of recognition down also to 0.5 ng glycoprotein. This imaging method was hence considered a new tool for analyzing glycoproteins.

Graphical abstract: Surface plasmon resonance imaging of limited glycoprotein samples

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Article information

DOI
https://doi.org/10.1039/B804235G
Article type
Paper
Submitted
11 Mar 2008
Accepted
22 Jul 2008
First published
30 Jul 2008

Analyst, 2008,133, 1268-1273

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Surface plasmon resonance imaging of limited glycoprotein samples

W. Liu, Y. Chen and M. Yan, Analyst, 2008, 133, 1268 DOI: 10.1039/B804235G

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