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Conformational analysis of the blue-light sensing protein YtvA reveals a competitive interface for LOV—LOV dimerization and interdomain interactions

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Abstract

The Bacillus subtilis protein YtvA is related to plant phototropins in that it senses UVA—blue-light by means of the flavin binding LOV domain, linked to a nucleotide-binding STAS domain. The structural basis for interdomain interactions and functional regulation are not known. Here we report the conformational analysis of three YtvA constructs, by means of size exclusion chromatography, circular dichroism (CD) and molecular docking simulations. The isolated YtvA-LOV domain (YLOV, aa 25–126) has a strong tendency to dimerize, prevented in full-length YtvA, but still observed in YLOV carrying the N-terminal extension (N-YLOV, aa 1–126). The analysis of CD data shows that both the N-terminal cap and the linker region (aa 127–147) between the LOV and the STAS domain are helical and that the central β-scaffold is distorted in the LOV domains dimers. The involvement of the central β-scaffold in dimerization is supported by docking simulation of the YLOV dimer and the importance of this region is highlighted by light-induced conformational changes, emerging from the CD data analysis. In YtvA, the β-strand fraction is notably less distorted and distinct light-driven changes in the loops/turn fraction are detected. The data uncover a common surface for LOV–LOV and intraprotein interaction, involving the central β-scaffold, and offer hints to investigate the molecular basis of light-activation and regulation in LOV proteins.

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Authors and Affiliations

  1. Dept. of Physics, University of Parma, via G.P. Usberti 7/A, 43100-, Parma, Italy

    Valentina Buttani & Aba Losi

  2. CNR-INFM, Parma, Italy

    Aba Losi

  3. Institut für Molekulare Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, Forschungszentrum Jülich, D-52426, Jülich, Germany

    Thorsten Eggert, Ulrich Krauss & Karl-Erich Jaeger

  4. Max-Planck-Institut für Bioanorganische Chemie, Stiftstr. 34-36, 45470, Mülheim, Germany

    Zhen Cao & Wolfgang Gärtner

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  1. Valentina Buttani
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  2. Aba Losi
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† Electronic supplementary information (ESI) available: Calculation of accessible and buried surface areas using the VADAR tool. See DOI:10.1039/b610375h

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Buttani, V., Losi, A., Eggert, T. et al. Conformational analysis of the blue-light sensing protein YtvA reveals a competitive interface for LOV—LOV dimerization and interdomain interactions. Photochem Photobiol Sci 6, 41–49 (2007). https://doi.org/10.1039/b610375h

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