Issue 10, 2005

Dynamic proteinadsorption in microchannels by “stop-flow” and continuous flow

Abstract

This work addresses two ways of loading proteins on microchannel surfaces for immunoassay applications: the “stop-flow” and the continuous flow processes. The “stop-flow” method consists of successive static incubation periods where the bulk solution depletes upon the adsorption process. In the present paper, a multi-step “stop-flow” protein coating is studied and compared to a coating under continuous flow conditions. For the “stop-flow”, a non-dimensional parameter is here introduced, indicating the adsorbing capacity of the system, by which it is possible to calculate the number of loads necessary to reach the optimum coverage. For the continuous flow, the effects on the adsorption of the kinetic rates, flow velocity and wall capacity have been considered. This study shows the importance of a careful choice of the fluid velocity to minimise the sample waste. For diffusion controlled and kinetics controlled processes, two flow velocity criteria are provided in order to obtain the best possible coverage, with the same amount of sample as with the “stop-flow”.

Graphical abstract: Dynamic protein adsorption in microchannels by “stop-flow” and continuous flow

Supplementary files

Article information

Article type
Paper
Submitted
24 Apr 2005
Accepted
04 Jul 2005
First published
11 Aug 2005

Lab Chip, 2005,5, 1096-1103

Dynamic protein adsorption in microchannels by “stop-flow” and continuous flow

A. Lionello, J. Josserand, H. Jensen and H. H. Girault, Lab Chip, 2005, 5, 1096 DOI: 10.1039/B506009E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements