Issue 27, 2005
From the journal:

Chemical Communications

Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS

Jun Zhe Min,a   Toshimasa Toyo'oka,*a   Masaru Katoa  and  Takeshi Fukushimaa  

* Corresponding authors

a Department of Analytical Chemistry, School of Pharmaceutical Sciences, and COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, Japan
E-mail: toyooka@ys2.u-shizuoka-ken.ac.jp
Fax: +81-54-264-5593
Tel: +81-54-264-5656

Abstract

The resolution of asparagine-type oligosaccharides in glycoproteins was carried out by combination of the transglycosylation reaction and CE-TOF-MS. The oligosaccharides enzymatically transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M. The resulting fluorescent-oligosaccharides were separated by CE and detected by TOF-MS. Disialo-Asn was successfully identified by the proposed procedure. Application to oligosaccharides in ovalbumin was also described in this communication.

Graphical abstract: Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS

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Article information

DOI
https://doi.org/10.1039/B505744B
Article type
Communication
Submitted
26 Apr 2005
Accepted
16 May 2005
First published
09 Jun 2005

Chem. Commun., 2005, 3484-3486

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Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS

J. Z. Min, T. Toyo'oka, M. Kato and T. Fukushima, Chem. Commun., 2005, 3484 DOI: 10.1039/B505744B

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