Issue 4, 2003
From the journal:

Analyst

Fluorescence polarization spectroscopy in protein analysis

Tina L. Manna  and  Ulrich J. Krull*a  

* Corresponding authors

a Chemical Sensors Group, Department of Chemistry, University of Toronto at Mississauga, 3359 Mississauga Road North, Mississauga, ON, Canada

Abstract

Information about concentration, molecular structure, binding events, and motion can be obtained using fluorescence spectroscopy methods. Fluorescence polarization spectroscopy is one such method, which studies the relationship between the polarization of light that is used for excitation and light that is subsequently detected from fluorescence. The extent of change of polarization between excitation and emission can be used to study physical processes such as rotational diffusion, extent of denaturation, and orientation at surfaces. In this article Mann and Krull describe the underlying principles to the technique and show how fluorescence polarization spectroscopy has contributed to protein analysis.

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Article information

DOI
https://doi.org/10.1039/B300873H
Article type
Education
First published
02 Apr 2003

Analyst, 2003,128, 313-317

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Fluorescence polarization spectroscopy in protein analysis

T. L. Mann and U. J. Krull, Analyst, 2003, 128, 313 DOI: 10.1039/B300873H

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