Issue 3, 2001
From the journal:

Analyst

Blood platelet adhesion to

Biljana A. Cavic,a   John Freedman,b   Zeynep Morel,a   Meera Mody,b   Margaret L. Rand,c   David C. Stonea  and  Michael Thompson*a  

* Corresponding authors

a Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada

b Department of Medicine, St. Michael’s Hospital, 30 Bond Street, Toronto, Ontario, Canada

c Division of Haematology/Oncology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada

Abstract

The attachment of blood platelets to the surface of bare and protein-coated thickness–shear mode acoustic wave devices operating in a flow-through configuration has been studied. Platelets in washed form bind to the gold electrodes of such sensors, but the resulting frequency shifts are far less than predicted by the conventional mass-based model of device operation. Adherence to albumin and various types of collagen can be produced by on-line introduction of protein or by a pre-coating strategy. Differences in attachment of platelets to collagen types I and IV and the Horm variety can be detected. Platelets attached to collagen yield an interesting delayed, but reversible signal on exposure to a flowing medium of low pH. Scanning electron microscopy of sensor surfaces at various time points in this experiment reveals that originally intact platelets are eventually destroyed by the high acidity of the medium. The reversible frequency is attributed to the presence of removable platelet granular components at the sensor–liquid interface.

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Article information

DOI
https://doi.org/10.1039/B009254L
Article type
Paper
Submitted
17 Nov 2000
Accepted
20 Dec 2000
First published
20 Feb 2001

Analyst, 2001,126, 342-348

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Blood platelet adhesion to protein studied by on-line acoustic wave sensor

B. A. Cavic, J. Freedman, Z. Morel, M. Mody, M. L. Rand, D. C. Stone and M. Thompson, Analyst, 2001, 126, 342 DOI: 10.1039/B009254L

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