Issue 3, 2001

Small volume bead assay for ovalbumin with electrochemical detection

Abstract

A bead based sandwich enzyme immunoassay coupled to electrochemical detection for ovalbumin has been developed. The enzyme label alkaline phosphatase was used to convert the substrate 4-aminophenyl phosphate to electroactive product 4-aminophenol. The detection was done in a microdrop by continuously monitoring the enzyme turnover with a rotating disk electrode. This reduces dilution of the enzyme product, a key to achieving low detection limits. The assay developed has a detection limit of 0.1 ng ml−1. Assay sensitivity in complex matrices such as food and serum was compared.

Article information

Article type
Paper
Submitted
18 Aug 2000
Accepted
05 Dec 2000
First published
30 Jan 2001

Analyst, 2001,126, 337-341

Small volume bead assay for ovalbumin with electrochemical detection

S. Purushothama, S. Kradtap, C. A. Wijayawardhana, H. B. Halsall and W. R. Heineman, Analyst, 2001, 126, 337 DOI: 10.1039/B006798I

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