Issue 39, 2020
From the journal:

Soft Matter

Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy†

Hammad A. Faizi,ab   Cody J. Reeves,c   Vasil N. Georgiev,b   Petia M. Vlahovska ORCID logo *ac  and  Rumiana Dimova ORCID logo *b  

* Corresponding authors

a Department of Mechanical Engineering, McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60208, USA
E-mail: Petia.Vlahovska@northwestern.edu

b Max Planck Institute of Colloids and Interfaces, Science Park Golm, 14424 Potsdam, Germany
E-mail: Rumiana.Dimova@mpikg.mpg.de

c Department of Engineering Sciences and Applied Mathematics, McCormick School of Engineering and Applied Science, Northwestern University, Evanston, Illinois 60208, USA

Abstract

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

Graphical abstract: Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

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Article information

DOI
https://doi.org/10.1039/D0SM00943A
Article type
Communication
Submitted
20 May 2020
Accepted
16 Sep 2020
First published
17 Sep 2020
This article is Open Access
Creative Commons BY license

Soft Matter, 2020,16, 8996-9001

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Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

H. A. Faizi, C. J. Reeves, V. N. Georgiev, P. M. Vlahovska and R. Dimova, Soft Matter, 2020, 16, 8996 DOI: 10.1039/D0SM00943A

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