miR-122 direct detection in human serum by time-gated fluorescence imaging

Emilio Garcia-Fernandez; M. Carmen Gonzalez-Garcia; Salvatore Pernagallo; Maria J. Ruedas-Rama; Mario A. Fara; Francisco J. López-Delgado; James W. Dear; Hugh Ilyine; Cristina Ress; Juan J. Díaz-Mochón; Angel Orte

doi:10.1039/C9CC08069D

miR-122 direct detection in human serum by time-gated fluorescence imaging†

Emilio Garcia-Fernandez,

^a M. Carmen Gonzalez-Garcia,^a Salvatore Pernagallo,^bc Maria J. Ruedas-Rama,

^a Mario A. Fara,^b Francisco J. López-Delgado,

^b James W. Dear,^d Hugh Ilyine,^c Cristina Ress,^e Juan J. Díaz-Mochón

^bfg and Angel Orte

*^a

Author affiliations

* Corresponding authors

^a Departamento de Fisicoquimica. Unidad de Excelencia de Química aplicada a Biomedicina y Medioambiente, Facultad de Farmacia, Universidad de Granada, Campus de Cartuja s/n, 18071-Granada, Spain
E-mail: angelort@ugr.es

^b DestiNA Genomica S.L., Parque Tecnológico Ciencias de la Salud (PTS), Av. de la Innovación 1, Edificio BIC, Armilla, Granada, Spain

^c DestiNA Genomics Ltd, 7-11 Melville St, Edinburgh EH3 7PE, UK

^d Centre for Cardiovascular Science, University of Edinburgh, The Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh, UK

^e Optoi Microelectronics, Via Vienna n°8, Trento, 38121 Gardolo, Italy

^f GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, Av. de la Ilustración, 114, 18016-Granada, Spain

^g Departamento de Quimica Farmaceutica y Organica, Facultad de Farmacia, Universidad de Granada, Campus de Cartuja s/n, 18071-Granada, Spain

Abstract

A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.

Chemical Communications

miR-122 direct detection in human serum by time-gated fluorescence imaging†

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miR-122 direct detection in human serum by time-gated fluorescence imaging

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