Issue 48, 2017, Issue in Progress
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RSC Advances

Desorption electrospray ionization mass spectrometry imaging reveals chemical defense of Burkholderia seminalis against cacao pathogens†

F. D. S. Araújo, ORCID logo *a   R. L. Vieira,a   E. P. L. Molano,b   H. J. Máximo,c   R. J. D. Dalio,c   P. H. Vendramini,a   W. L. Araújod  and  M. N. Eberlina  

* Corresponding authors

a ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, POB 6154, 13084-970, Campinas, SP, Brazil
E-mail: fdsaraujo@gmail.com

b Genomic and Expression Laboratory, Department of Genetics, Evolution and Bio-agents, Institute of Biology, University of Campinas, 13083-970, Campinas, SP, Brazil

c Biotechnology Laboratory, Centro de Citricultura Sylvio Moreira/Agronomic Institute, 13490-970, Cordeirópolis, SP, Brazil

d Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, 05508-900, São Paulo, SP, Brazil

Abstract

Fungal and oomycete diseases are responsible for significant economic losses of cacao crops worldwide. Such diseases are caused mainly by the fungus Moniliophthora perniciosa, and the oomycetes Phytophthora citrophtora, P. capsici, and P. palmivora. The endophytic bacterium Burkholderia seminalis has showed antagonistic action against several plant pathogens. Herein, desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was applied to map spatial metabolic distributions from B. seminalis and cacao pathogen monocultures, as well as to monitor interkingdom interactions of the endophytic bacterium with the fungus and oomycetes. DESI-MSI was successful in the direct analysis of fungus and oomycete pathogens on dehydrated agar plate, detecting several phospholipids and other unidentified metabolites. Chemical defense of B. seminalis against cacao pathogens was evidenced by the detection of partially diffuse rhamnolipid, which probably act in synergism with other unidentified diffuse metabolites.

Graphical abstract: Desorption electrospray ionization mass spectrometry imaging reveals chemical defense of Burkholderia seminalis against cacao pathogens

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Article information

DOI
https://doi.org/10.1039/C7RA03895J
Article type
Paper
Submitted
06 Apr 2017
Accepted
22 May 2017
First published
09 Jun 2017
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2017,7, 29953-29958

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Desorption electrospray ionization mass spectrometry imaging reveals chemical defense of Burkholderia seminalis against cacao pathogens

F. D. S. Araújo, R. L. Vieira, E. P. L. Molano, H. J. Máximo, R. J. D. Dalio, P. H. Vendramini, W. L. Araújo and M. N. Eberlin, RSC Adv., 2017, 7, 29953 DOI: 10.1039/C7RA03895J

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