Issue 107, 2016, Issue in Progress

Functions of the Mg–HA coating on carbon/carbon composite surface to promote the proliferation and osteogenic differentiation of mBMSCs

Abstract

This study aims to evaluate the functions of the Mg–hydroxyapatite (Mg–HA) bio-coating on carbon/carbon composite (C/C) surface to promote the proliferation and osteogenic differentiation of bone mesenchymal stem cell (BMSCs). The Mg–HA-coated carbon/carbon composite was placed into a medium. The change of ion concentration in the solution and formation of bone-like apatite on the composite surface were observed. Thereafter, BMSCs of mice (mBMSCs) were cultivated on the coating surface for 4, 8, 24, 48, and 72 h. Finally, gene changes (i.e., Alp, OCN, Osx, and Runx2) related to cell proliferation and osteogenic differentiation were observed. The Mg–HA coating can form a bone-like apatite coating of high Mg concentration in the medium. After mBMSCs were cultivated on the Mg–HA coating for 72 h, mRNA of Alp, Osx, and Runx2 in Mg–HA was higher than that in the HA group (P < 0.05 or P < 0.001). Accordingly, mRNA of OCN did not show significant difference in different time points between the two groups (P > 0.05). The Mg coating can significantly promote the proliferation and osteogenic differentiation of mBMSCs.

Graphical abstract: Functions of the Mg–HA coating on carbon/carbon composite surface to promote the proliferation and osteogenic differentiation of mBMSCs

Article information

Article type
Paper
Submitted
14 Aug 2016
Accepted
28 Oct 2016
First published
03 Nov 2016

RSC Adv., 2016,6, 105056-105062

Functions of the Mg–HA coating on carbon/carbon composite surface to promote the proliferation and osteogenic differentiation of mBMSCs

X. Xinbo, N. Xinye and Z. Dong, RSC Adv., 2016, 6, 105056 DOI: 10.1039/C6RA20481C

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