Issue 16, 2016
From the journal:

RSC Advances

Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine†

Alejandro Gutiérrez,a   Fabiana A. Gutierrez,a   Marcos Eguílaz,a   José M. González-Domínguez,‡b   Javier Hernández-Ferrer,b   Alejandro Ansón-Casaos,b   María T. Martínez*b  and  Gustavo A. Rivas*a  

* Corresponding authors

a INFIQC, Departamento de Físico Química, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina
E-mail: grivas@fcq.unc.edu.ar

b Instituto de Carboquímica (CSIC), C/ Miguel Luesma Castán 4, E-50018 Zaragoza, Spain
E-mail: mtmartinez@icb.csic.es

Abstract

This work reports the synthesis and characterization of single-walled carbon nanotubes (SWCNT) covalently functionalized with L-lysine (Lys) and the analytical performance of glassy carbon electrodes (GCE) modified with a dispersion of SWCNT-Lys (GCE/SWCNT-Lys) for the highly sensitive quantification of guanine, adenine and 8-hydroxy-2′-deoxyguanosine. Detection limits of 75, 195 and 97 nM were obtained for guanine, adenine and 8-hydroxy-2′-deoxyguanosine, respectively by voltammetric adsorptive stripping with medium exchange. GCE/SWCNT-Lys was successfully used for the detection of adenine and guanine oxidation after adsorption of salmon sperm-double stranded DNA. A clear definition of 8-hydroxy-2′-deoxyguanosine oxidation signal is observed even in the presence of large excess of guanine, adenine or salmon sperm-double stranded DNA.

Graphical abstract: Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine

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Article information

DOI
https://doi.org/10.1039/C5RA22556F
Article type
Paper
Submitted
28 Oct 2015
Accepted
18 Jan 2016
First published
21 Jan 2016

RSC Adv., 2016,6, 13469-13477

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Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine

A. Gutiérrez, F. A. Gutierrez, M. Eguílaz, J. M. González-Domínguez, J. Hernández-Ferrer, A. Ansón-Casaos, M. T. Martínez and G. A. Rivas, RSC Adv., 2016, 6, 13469 DOI: 10.1039/C5RA22556F

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