Issue 2, 2016

TiO2 nanocrystals – assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods

Abstract

In the present study, the possibility for the application of TiO2 nanocrystals of various shapes and sizes, for substrate-assisted laser desorption and ionization time-of-flight mass spectrometric (SALDI TOF MS) quantitative analysis of small molecules (steroid hormones, amino acids and saccharides) was investigated. Parameters, such as homogeneity of the substrate/analyte distribution, reproducibility of the measurements, within-day, and day-to-day repeatability, were determined. The homogeneity of different nanocrystal/analyte combinations on the target plate were compared based on the signal-to-noise values of several analyte signals. Obtained results show that all TiO2 nanocrystals, regardless of their shape, have great potential for the detection and determination of steroid hormones, amino acids and saccharides with good analytical parameters and detection limits. On the other hand, the reproducibility of the S/N ratio and detectability of the analytes recorded in various modes differ depending on the substrate. All examined molecules were detectable in negative ion mode with TiO2 NTs, in contrast to all other organic matrices and substrates, and the best reproducibility was obtained with the larger nanocrystals, TiO2 PNSs and TiO2 NTs, making them good candidates for the quantitative determination of small molecules.

Graphical abstract: TiO2 nanocrystals – assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods

Supplementary files

Article information

Article type
Paper
Submitted
28 Sep 2015
Accepted
02 Dec 2015
First published
03 Dec 2015

RSC Adv., 2016,6, 1027-1036

Author version available

TiO2 nanocrystals – assisted laser desorption and ionization time-of-flight mass spectrometric analysis of steroid hormones, amino acids and saccharides. Validation and comparison of methods

I. Popović, M. Nešić, M. Vranješ, Z. Šaponjić and M. Petković, RSC Adv., 2016, 6, 1027 DOI: 10.1039/C5RA20042C

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