Gp10 based-thioetherification (10BASEd-T) on a displaying library peptide of bacteriophage T7

Keisuke Fukunaga; Takaaki Hatanaka; Yuji Ito; Masumi Taki

doi:10.1039/C3MB70379G

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Gp10 based-thioetherification (10BASE_d-T) on a displaying library peptide of bacteriophage T7†

Keisuke Fukunaga,^a Takaaki Hatanaka,^b Yuji Ito^b and Masumi Taki*^a

Author affiliations

* Corresponding authors

^a Department of Engineering Science, Bioscience and Technology Program, The Graduate School of Informatics and Engineering, The University of Electro-Communications (UEC), 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan
E-mail: taki@pc.uec.ac.jp
Tel: +81-42-442-5980

^b Department of Chemistry and Bioscience, Graduate School of Science and Engineering, Kagoshima University, 1-21-35 Korimoto, Kagoshima, Kagoshima 890-0065, Japan

Abstract

The site-specific introduction of a haloacetamide derivative into a designated cysteine on a displaying peptide on a capsid protein (gp10) of bacteriophage T7 has been achieved. This easiest gp10-based thioetherification (10BASE_d-T) is carried out in one-pot without side reactions or loss of phage infectivity.

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Article information

DOI: https://doi.org/10.1039/C3MB70379G
Article type: Communication
Submitted: 02 Sep 2013
Accepted: 16 Sep 2013
First published: 27 Sep 2013

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Mol. BioSyst., 2013,9, 2988-2991

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Gp10 based-thioetherification (10BASE_d-T) on a displaying library peptide of bacteriophage T7

K. Fukunaga, T. Hatanaka, Y. Ito and M. Taki, Mol. BioSyst., 2013, 9, 2988 DOI: 10.1039/C3MB70379G

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