Issue 19, 2006
From the journal:

Chemical Communications

Synthesis and single enzyme activity of a clicked lipase–BSA hetero-dimer†

Nikos S. Hatzakis,a   Hans Engelkamp,a   Kelly Velonia,b   Johan Hofkens,c   Peter C. M. Christianen,a   Allan Svendsen,d   Shamkantr A. Patkar,d   Jesper Vind,a   Jan C. Maan,a   Alan E. Rowan*a  and  Roeland J. M. Nolte*a  

* Corresponding authors

a Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, Nijmegen, The Netherlands
E-mail: A.Rowan@science.ru.nl; R.Nolte@science.ru.nl
Fax: (+31) 24 365 2929
Tel: +31 (0)24 36 52323

b Department of Organic Chemistry, University of Geneva, 30 quai Ernest Ansermet, Geneva 4, Switzerland

c Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium

d NoVo-Nordisk a/s, NoVo Alle, Bagsvaerd, Denmark

Abstract

Click chemistry is used to construct a novel lipase–BSA hetero-dimer, in which the latter protein acts as a foot enabling the anchoring of the enzyme onto the surface for single enzyme studies.

Graphical abstract: Synthesis and single enzyme activity of a clicked lipase–BSA hetero-dimer

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Article information

DOI
https://doi.org/10.1039/B516551B
Article type
Communication
Submitted
22 Nov 2005
Accepted
19 Jan 2006
First published
09 Feb 2006

Chem. Commun., 2006, 2012-2014

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Synthesis and single enzyme activity of a clicked lipase–BSA hetero-dimer

N. S. Hatzakis, H. Engelkamp, K. Velonia, J. Hofkens, P. C. M. Christianen, A. Svendsen, S. A. Patkar, J. Vind, J. C. Maan, A. E. Rowan and R. J. M. Nolte, Chem. Commun., 2006, 2012 DOI: 10.1039/B516551B

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