Issue 12, 2008
From the journal:

Lab on a Chip

Integration of semiconductor quantum dots into nano-bio-chip systems for enumeration of CD4+ T cell counts at the point-of-need†‡

Jesse V. Jokerst,a   Pierre N. Floriano,a   Nicolaos Christodoulides,a   Glennon W. Simmonsa  and  John T. McDevitt*abc  

* Corresponding authors

a Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX, USA
E-mail: mcdevitt@mail.utexas.edu
Fax: +512-232-7052
Tel: +512-471-0046

b Center for Nano and Molecular Science and Technology, The University of Texas at Austin, Austin, TX, USA

c Texas Materials Institute, The University of Texas at Austin, Austin, TX, USA

Abstract

Recent humanitarian efforts have led to the widespread release of antiretroviral drugs for the treatment of the more than 33 million HIV afflicted people living in resource-scarce settings. Here, the enumeration of CD4+ T lymphocytes is required to establish the level at which the immune system has been compromised. The gold standard method used in developed countries, based on flow cytometry, though widely accepted and accurate, is precluded from widespread use in resource-scarce settings due to its high expense, high technical requirements, difficulty in operation-maintenance and the lack of portability for these sophisticated laboratory-confined systems. As part of continuing efforts to develop practical diagnostic instrumentation, the integration of semiconductor nanocrystals (quantum dots, QDs) into a portable microfluidic-based lymphocyte capture and detection device is completed. This integrated system is capable of isolating and counting selected lymphocyte sub-populations (CD3+CD4+) from whole blood samples. By combining the unique optical properties of the QDs with the sample handling capabilities and cost effectiveness of novel microfluidic systems, a practical, portable lymphocyte measurement modality that correlates nicely with flow cytometry (R2 = 0.97) has been developed. This QD-based system reduces the optical requirements significantly relative to molecular fluorophores and the mini-CD4 counting device is projected to be suitable for use in both point-of-need and resource-scarce settings.

Graphical abstract: Integration of semiconductor quantum dots into nano-bio-chip systems for enumeration of CD4+ T cell counts at the point-of-need

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Article information

DOI
https://doi.org/10.1039/B817116E
Article type
Paper
Submitted
30 Sep 2008
Accepted
15 Oct 2008
First published
05 Nov 2008

Lab Chip, 2008,8, 2079-2090

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Integration of semiconductor quantum dots into nano-bio-chip systems for enumeration of CD4+ T cell counts at the point-of-need

J. V. Jokerst, P. N. Floriano, N. Christodoulides, G. W. Simmons and J. T. McDevitt, Lab Chip, 2008, 8, 2079 DOI: 10.1039/B817116E

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