Issue 14, 2016
From the journal:

Analyst

Toward food analytics: fast estimation of lycopene and β-carotene content in tomatoes based on surface enhanced Raman spectroscopy (SERS)†

Andreea Ioana Radu,ab   Oleg Ryabchykov,ab   Thomas Wilhelm Bocklitz, ORCID logo ab   Uwe Huebner,b   Karina Weber,ab   Dana Cialla-May*ab  and  Jürgen Poppab  

* Corresponding authors

a Friedrich Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07745 Jena, Germany

b Leibniz Institute of Photonic Technology Jena, Albert-Einstein-Str. 9, 07745 Jena, Germany
E-mail: dana.cialla-may@uni-jena.de

Abstract

Carotenoids are molecules that play important roles in both plant development and in the well-being of mammalian organisms. Therefore, various studies have been performed to characterize carotenoids’ properties, distribution in nature and their health benefits upon ingestion. Nevertheless, there is a gap regarding a fast detection of them at the plant phase. Within this contribution we report the results obtained regarding the application of surface enhanced Raman spectroscopy (SERS) toward the differentiation of two carotenoid molecules (namely, lycopene and β-carotene) in tomato samples. To this end, an e-beam lithography (EBL) SERS-active substrate and a 488 nm excitation source were employed, and a relevant simulated matrix was prepared (by mixing the two carotenoids in defined percentages) and measured. Next, carotenoids were extracted from tomato plants and measured as well. Finally, a combination of principal component analysis and partial least squares regression (PCA-PLSR) was applied to process the data, and the obtained results were compared with HPLC measurements of the same extracts. A good agreement was obtained between the HPLC and the SERS results for most of the tomato samples.

Graphical abstract: Toward food analytics: fast estimation of lycopene and β-carotene content in tomatoes based on surface enhanced Raman spectroscopy (SERS)

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Article information

DOI
https://doi.org/10.1039/C6AN00390G
Article type
Paper
Submitted
16 Feb 2016
Accepted
12 May 2016
First published
12 May 2016
This article is Open Access
Creative Commons BY-NC license

Analyst, 2016,141, 4447-4455

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Toward food analytics: fast estimation of lycopene and β-carotene content in tomatoes based on surface enhanced Raman spectroscopy (SERS)

A. I. Radu, O. Ryabchykov, T. W. Bocklitz, U. Huebner, K. Weber, D. Cialla-May and J. Popp, Analyst, 2016, 141, 4447 DOI: 10.1039/C6AN00390G

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